The University of Southampton
University of Southampton Institutional Repository

Immunogenetic analysis of the immune response to pneumococcal polysaccharide

Immunogenetic analysis of the immune response to pneumococcal polysaccharide
Immunogenetic analysis of the immune response to pneumococcal polysaccharide
Pneumococcal serotype-specific anti-capsular polysaccharide antibodies protect against invasive pneumococcal disease. Within an individual the diversity of these antibodies is limited. To evaluate the repertoire of antibodies to pneumococcus and determine whether oligoclonality is seen both between serotypes and between individuals, we sampled the B cell repertoire induced by polysaccharide and conjugate vaccine in adult volunteers. Fifteen hybridomas secreting pneumococcus-specific monoclonal antibodies were generated from five volunteers. Ten were isotype switched, six were IgG2 and four were IgA. These included two isotype switch variants of the same clone. V(H)3 and V(kappa)2 were used by 10/15 and 7/13 of the sequenced clones, respectively, with identical genes, V(H)3-48 and V(kappa)2-A17 used by a number of volunteers to a variety of serotypes. VDJ junctional characteristics and complementarity-determining region (CDR) 3 length were variable. High levels of somatic mutation in CDR1 and 2, inconsistent with a primary response, were found in 10/11 of the isotype-switched antibodies, including those induced by plain polysaccharide antigens. These data suggest that wild-type infection or nasopharyngeal carriage of Streptococcus pneumoniae in adults may induce memory and the response to subsequent immunization with plain polysaccharide or conjugate pneumococcal vaccines may have the characteristics of a secondary response.
0014-2980
1214-1223
Baxendale, H.E.
68988edd-38cc-4bf5-90fc-498e965f54cb
Davis, Z.
b106965c-11bb-4133-98f0-540894dd11a9
White, H.N.
437b0a3f-0e13-4734-8e8e-d0fc0a98e7af
Spellerberg, M.B.
f68fa135-0aef-4be2-826f-9ed21d18785b
Stevenson, F.K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Goldblatt, D.
3f9d26e2-06f2-4778-a3d8-1a88bbed9c0f
Baxendale, H.E.
68988edd-38cc-4bf5-90fc-498e965f54cb
Davis, Z.
b106965c-11bb-4133-98f0-540894dd11a9
White, H.N.
437b0a3f-0e13-4734-8e8e-d0fc0a98e7af
Spellerberg, M.B.
f68fa135-0aef-4be2-826f-9ed21d18785b
Stevenson, F.K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Goldblatt, D.
3f9d26e2-06f2-4778-a3d8-1a88bbed9c0f

Baxendale, H.E., Davis, Z., White, H.N., Spellerberg, M.B., Stevenson, F.K. and Goldblatt, D. (2000) Immunogenetic analysis of the immune response to pneumococcal polysaccharide. European Journal of Immunology, 30 (4), 1214-1223.

Record type: Article

Abstract

Pneumococcal serotype-specific anti-capsular polysaccharide antibodies protect against invasive pneumococcal disease. Within an individual the diversity of these antibodies is limited. To evaluate the repertoire of antibodies to pneumococcus and determine whether oligoclonality is seen both between serotypes and between individuals, we sampled the B cell repertoire induced by polysaccharide and conjugate vaccine in adult volunteers. Fifteen hybridomas secreting pneumococcus-specific monoclonal antibodies were generated from five volunteers. Ten were isotype switched, six were IgG2 and four were IgA. These included two isotype switch variants of the same clone. V(H)3 and V(kappa)2 were used by 10/15 and 7/13 of the sequenced clones, respectively, with identical genes, V(H)3-48 and V(kappa)2-A17 used by a number of volunteers to a variety of serotypes. VDJ junctional characteristics and complementarity-determining region (CDR) 3 length were variable. High levels of somatic mutation in CDR1 and 2, inconsistent with a primary response, were found in 10/11 of the isotype-switched antibodies, including those induced by plain polysaccharide antigens. These data suggest that wild-type infection or nasopharyngeal carriage of Streptococcus pneumoniae in adults may induce memory and the response to subsequent immunization with plain polysaccharide or conjugate pneumococcal vaccines may have the characteristics of a secondary response.

Full text not available from this repository.

More information

Published date: April 2000

Identifiers

Local EPrints ID: 157809
URI: https://eprints.soton.ac.uk/id/eprint/157809
ISSN: 0014-2980
PURE UUID: 0490c874-190e-42c8-8091-4b918af3724d
ORCID for F.K. Stevenson: ORCID iD orcid.org/0000-0002-0933-5021

Catalogue record

Date deposited: 06 Jul 2010 15:48
Last modified: 06 Jun 2018 13:01

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×