Surfactant protein D binds genomic DNA and apoptotic cells, and enhances their clearance, in vivo
Surfactant protein D binds genomic DNA and apoptotic cells, and enhances their clearance, in vivo
Collectins, such as surfactant protein D (SP-D), bind apoptotic cells; however, the ligands that they recognize on these cells are unknown. We hypothesized that SP-D binds to the DNA present on these cells. We show that SP-D binds and aggregates mouse alveolar macrophage DNA effectively. Alveolar macrophages of SP-D((-/-)) mice contained more nicked DNA than those of SP-A((-/-)) and wild type mice. Our results also suggest that carbohydrate recognition domains of SP-D may recognize DNA present on the apoptotic cells. Therefore, cell-surface DNA could be a ligand for recognition of apoptotic cells by collectins.
471-475
Palaniyar, Nades
262890ae-b2b1-4f9b-8129-08f8bcd6a399
Clark, Howard
a5fd860a-269b-4e37-9075-73f94eb00471
Nadesalingam, Jeya
5530d9f4-bb87-40ce-a3b1-34352d245a47
Hawgood, Samuel
5e406d87-8445-448a-a068-b693d061d6dd
Reid, Kenneth B.M.
74c6c3e3-d682-446d-b159-b635e6056e4e
December 2003
Palaniyar, Nades
262890ae-b2b1-4f9b-8129-08f8bcd6a399
Clark, Howard
a5fd860a-269b-4e37-9075-73f94eb00471
Nadesalingam, Jeya
5530d9f4-bb87-40ce-a3b1-34352d245a47
Hawgood, Samuel
5e406d87-8445-448a-a068-b693d061d6dd
Reid, Kenneth B.M.
74c6c3e3-d682-446d-b159-b635e6056e4e
Palaniyar, Nades, Clark, Howard, Nadesalingam, Jeya, Hawgood, Samuel and Reid, Kenneth B.M.
(2003)
Surfactant protein D binds genomic DNA and apoptotic cells, and enhances their clearance, in vivo.
Annals of the New York Academy of Sciences, 1010, .
Abstract
Collectins, such as surfactant protein D (SP-D), bind apoptotic cells; however, the ligands that they recognize on these cells are unknown. We hypothesized that SP-D binds to the DNA present on these cells. We show that SP-D binds and aggregates mouse alveolar macrophage DNA effectively. Alveolar macrophages of SP-D((-/-)) mice contained more nicked DNA than those of SP-A((-/-)) and wild type mice. Our results also suggest that carbohydrate recognition domains of SP-D may recognize DNA present on the apoptotic cells. Therefore, cell-surface DNA could be a ligand for recognition of apoptotic cells by collectins.
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Published date: December 2003
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Local EPrints ID: 158233
URI: http://eprints.soton.ac.uk/id/eprint/158233
ISSN: 0077-8923
PURE UUID: d9e13b42-0e0a-462c-828f-ba0c011c9518
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Date deposited: 16 Jun 2010 15:38
Last modified: 08 Jan 2022 05:32
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Contributors
Author:
Nades Palaniyar
Author:
Howard Clark
Author:
Jeya Nadesalingam
Author:
Samuel Hawgood
Author:
Kenneth B.M. Reid
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