A recombinant fragment of human SP-D reduces allergic responses in mice sensitized to house dust mite allergens

Strong, P., Townsend, P., Mackay, Rosemarie, Reid, K.B.M. and Clark, H.W. (2003) A recombinant fragment of human SP-D reduces allergic responses in mice sensitized to house dust mite allergens. Clinical and Experimental Immunology, 134 (2), 181-7. (doi:10.1046/j.1365-2249.2003.02281.x).

Abstract

C57Bl6 mice sensitized to Dermatophagoides pteronyssinus and challenged with D. pteronyssinus allergen extract given intranasally followed by treatment with intranasal applications of a 60-kDa truncated, trimeric recombinant form of human SP-D (rfhSP-D) showed a significant reduction in serum IgE, IgG1, peripheral blood eosinophilia and airway hyperresponsiveness compared to saline or bovine serum albumin-treated controls.

Intracellular cytokine staining of lung and spleen homogenates showed increases in interleukin (IL)-12 production in lung tissue and normalization of IL-12 and interferon (IFN)-gamma in spleen tissue. In previous studies we demonstrated the effectiveness of native SP-D and rfhSP-D in down-regulating allergic responses to allergens of Aspergillus fumigatus. The results reported here indicate that rfhSP-D can suppress the development of allergic symptoms in sensitized mice challenged with allergens of the common house dust mite.

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Contributors

Author: Rosemarie Mackay

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