The University of Southampton
University of Southampton Institutional Repository

Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions

Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions
Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions
Pulmonary surfactant exists in the alveolus in several distinct subtypes that differ in their morphology, composition, and surface activity. Experiments by others have implicated a serine hydrolase in the production of the inactive small vesicular subtype of surfactant (N. J. Gross and R. M. Schultz. Biochim. Biophys. Acta 1044: 222-230, 1990). Our laboratory recently identified this enzyme in the rat as the serine carboxylesterase ES-2 [F. Barr, H. Clark, and S. Hawgood. Am. J. Physiol. 274 (Lung Cell. Mol. Physiol. 18): L404-L410, 1998]. In the present study, we determined the cellular sites of expression of ES-2 in rat lung using a digoxygenin-labeled ES-2 riboprobe. ES-2 mRNA was localized to type II cells and alveolar macrophages but not to Clara cells. Using a specific ES-2 antibody, we determined the protein distribution of ES-2 in the lung by immunohistochemistry, and it was found to be consistent with the sites of mRNA expression. Most of the ES-2 in rat bronchoalveolar lavage is in the surfactant-depleted supernatant, but ES-2 was also consistently localized to the small vesicular surfactant subfraction presumed to form as a consequence of conversion activity. These results are consistent with a role for endogenous lung ES-2 in surfactant metabolism.
1040-0605
L452-L458
Clark, H.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Allen, L.
dfec9b60-935b-4061-b010-679ed9a76b54
Collins, E.
cff2f5ef-c476-46c6-ad53-bc79fd5c94ea
Barr, F.
4a7ff0c5-1417-4787-93d6-13db432e930d
Dobbs, L.
90804e7e-04a3-4160-bb2a-05d1b3f5d180
Putz, G.
31920095-a0d6-43cf-9224-36336aa88ded
Goerke, J.
7101124b-1014-4b82-8f00-ab608ab1fc77
Hawgood, S.
2fce9ebe-495f-44fc-9e62-d7632dcafe9c
Clark, H.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Allen, L.
dfec9b60-935b-4061-b010-679ed9a76b54
Collins, E.
cff2f5ef-c476-46c6-ad53-bc79fd5c94ea
Barr, F.
4a7ff0c5-1417-4787-93d6-13db432e930d
Dobbs, L.
90804e7e-04a3-4160-bb2a-05d1b3f5d180
Putz, G.
31920095-a0d6-43cf-9224-36336aa88ded
Goerke, J.
7101124b-1014-4b82-8f00-ab608ab1fc77
Hawgood, S.
2fce9ebe-495f-44fc-9e62-d7632dcafe9c

Clark, H., Allen, L., Collins, E., Barr, F., Dobbs, L., Putz, G., Goerke, J. and Hawgood, S. (1999) Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions. American Journal of Physiology: Lung Cellular and Molecular Physiology, 276 (3), part 1, L452-L458.

Record type: Article

Abstract

Pulmonary surfactant exists in the alveolus in several distinct subtypes that differ in their morphology, composition, and surface activity. Experiments by others have implicated a serine hydrolase in the production of the inactive small vesicular subtype of surfactant (N. J. Gross and R. M. Schultz. Biochim. Biophys. Acta 1044: 222-230, 1990). Our laboratory recently identified this enzyme in the rat as the serine carboxylesterase ES-2 [F. Barr, H. Clark, and S. Hawgood. Am. J. Physiol. 274 (Lung Cell. Mol. Physiol. 18): L404-L410, 1998]. In the present study, we determined the cellular sites of expression of ES-2 in rat lung using a digoxygenin-labeled ES-2 riboprobe. ES-2 mRNA was localized to type II cells and alveolar macrophages but not to Clara cells. Using a specific ES-2 antibody, we determined the protein distribution of ES-2 in the lung by immunohistochemistry, and it was found to be consistent with the sites of mRNA expression. Most of the ES-2 in rat bronchoalveolar lavage is in the surfactant-depleted supernatant, but ES-2 was also consistently localized to the small vesicular surfactant subfraction presumed to form as a consequence of conversion activity. These results are consistent with a role for endogenous lung ES-2 in surfactant metabolism.

Full text not available from this repository.

More information

Published date: March 1999

Identifiers

Local EPrints ID: 158573
URI: http://eprints.soton.ac.uk/id/eprint/158573
ISSN: 1040-0605
PURE UUID: 17c10932-0892-4a03-b146-94b3a5952bda

Catalogue record

Date deposited: 14 Jul 2010 08:44
Last modified: 16 Jul 2019 23:55

Export record

Contributors

Author: H. Clark
Author: L. Allen
Author: E. Collins
Author: F. Barr
Author: L. Dobbs
Author: G. Putz
Author: J. Goerke
Author: S. Hawgood

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×