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Intranasal application of chitin micro-particles down-regulates symptoms of allergic hypersensitivity to Dermatophagoides pteronyssinus and Aspergillus umigatus in murine models of allergy

Intranasal application of chitin micro-particles down-regulates symptoms of allergic hypersensitivity to Dermatophagoides pteronyssinus and Aspergillus umigatus in murine models of allergy
Intranasal application of chitin micro-particles down-regulates symptoms of allergic hypersensitivity to Dermatophagoides pteronyssinus and Aspergillus umigatus in murine models of allergy
Background

Previous studies have demonstrated that chitin in the form of microparticles that can be phagocytosed is a potent macrophage stimulator and promotes a Th1 cytokine response and it has been shown that oral administration of chitin microparticles is effective in down-regulating serum IgE and lung eosinophilia in a mouse model of ragweed allergy. To date there have been no studies on the effectiveness of directly applying chitin microparticles to the respiratory tract as a treatment for allergic symptoms.

Objective

To test the effectiveness of chitin microparticles when given intranasally as a treatment for the symptoms of respiratory allergy and allergic asthma and to compare its effectiveness in two different mouse models of allergy, namely to Dermatophagoides pteronyssinus and Aspergillus fumigatus.

Results

The intranasal application of microgram doses of chitin microparticles is an effective treatment for reducing serum IgE and peripheral blood eosinophilia, airway hyper-responsiveness and lung inflammation in both allergy models results in elevation in Th1 cytokines IL-12, IFN-? and TNF-? and reduction in IL-4 production during allergen challenge.

Conclusion

Chitin microparticle suspensions have Th1 immunostimulatory properties and are effective when administered intranasally in mice. The stimulation of the nasal associated lymphoid tissue with chitin microparticles could offer a novel and natural approach to treating allergic disease in humans.
allergy, Aspergillus fumigatus, chitin, Der p, IFN-?, IL-12, immunotherapy, intranasal treatment
0954-7894
1794-1800
Strong, P.
4f2021be-1060-44b0-b384-4ab92c3d6f23
Clark, Howard
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Reid, K.
5c54fe9d-691a-445d-ab8f-e3e2e7676bb6
Strong, P.
4f2021be-1060-44b0-b384-4ab92c3d6f23
Clark, Howard
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Reid, K.
5c54fe9d-691a-445d-ab8f-e3e2e7676bb6

Strong, P., Clark, Howard and Reid, K. (2002) Intranasal application of chitin micro-particles down-regulates symptoms of allergic hypersensitivity to Dermatophagoides pteronyssinus and Aspergillus umigatus in murine models of allergy. Clinical & Experimental Allergy, 32 (12), 1794-1800. (doi:10.1046/j.1365-2222.2002.01551.x).

Record type: Article

Abstract

Background

Previous studies have demonstrated that chitin in the form of microparticles that can be phagocytosed is a potent macrophage stimulator and promotes a Th1 cytokine response and it has been shown that oral administration of chitin microparticles is effective in down-regulating serum IgE and lung eosinophilia in a mouse model of ragweed allergy. To date there have been no studies on the effectiveness of directly applying chitin microparticles to the respiratory tract as a treatment for allergic symptoms.

Objective

To test the effectiveness of chitin microparticles when given intranasally as a treatment for the symptoms of respiratory allergy and allergic asthma and to compare its effectiveness in two different mouse models of allergy, namely to Dermatophagoides pteronyssinus and Aspergillus fumigatus.

Results

The intranasal application of microgram doses of chitin microparticles is an effective treatment for reducing serum IgE and peripheral blood eosinophilia, airway hyper-responsiveness and lung inflammation in both allergy models results in elevation in Th1 cytokines IL-12, IFN-? and TNF-? and reduction in IL-4 production during allergen challenge.

Conclusion

Chitin microparticle suspensions have Th1 immunostimulatory properties and are effective when administered intranasally in mice. The stimulation of the nasal associated lymphoid tissue with chitin microparticles could offer a novel and natural approach to treating allergic disease in humans.

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More information

Published date: 2002
Keywords: allergy, Aspergillus fumigatus, chitin, Der p, IFN-?, IL-12, immunotherapy, intranasal treatment

Identifiers

Local EPrints ID: 158857
URI: http://eprints.soton.ac.uk/id/eprint/158857
ISSN: 0954-7894
PURE UUID: 0c32309d-3331-46dc-8d2a-70fd7b71ef72

Catalogue record

Date deposited: 24 Jun 2010 10:45
Last modified: 14 Mar 2024 01:52

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Contributors

Author: P. Strong
Author: Howard Clark
Author: K. Reid

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