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Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study

Clark, H.W. and Jacobson, W. (1991) Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study Journal of Obstetrics & Gynaecology, 11, (5), pp. 307-314.

Record type: Article

Abstract

Particles of pulmonary surfactant can be detected in
minute quantities of amniotic fluid; they appear as round
birefringent particles of 0.5-3 pm diameter with a characteristic cross of polarisation. Two hundred and five samples of amniotic fluid of various gestational ages were examined microscopically under polarised light. The number of birefringent particles observed per pI of amniotic fluid correlated well with the percentage of phosphatidyl-choline in the sample. There was also good agreement with palmitate determinations, surface tension measurements. the
1ecithin:sphingomyelin ratio and the clinical data. This technique is simple and rapid and does not require counting surfactant particles.

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Published date: 1991

Identifiers

Local EPrints ID: 158861
URI: http://eprints.soton.ac.uk/id/eprint/158861
ISSN: 0144-3615
PURE UUID: 141c1fdc-3409-465b-9784-87b60ba4253d

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Date deposited: 24 Jun 2010 09:28
Last modified: 18 Jul 2017 12:38

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Contributors

Author: H.W. Clark
Author: W. Jacobson

University divisions

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