Microfluidic systems for cell transfection using sonoporation and electroporation
Microfluidic systems for cell transfection using sonoporation and electroporation
Studies into sonoporation and electroporation have grown rapidly in biotechnology and medicine in recent years. This research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation system and electroporation system with certain biological cells. Ultrasonic standing waves, previously used for ultrasonic particle manipulation, have been used in the development of the sonoporation aspects of the system. MATLAB has also been used to analyse the required acoustic conditions within the chamber. Furthermore, the electroporation system makes use of a relatively simple circuit consisting of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The electroporation system has been designed, developed and tested. The system was evaluated with HeLa cells, THP-1 cells and plasmid DNA (pEGFP-N1) to evaluate transfection rates under a variety of sonoporation conditions. This study also determined cell viability under a range of sonoporation and electroporation conditions.
Rodamporn, Somphop
43aa084a-2a90-41de-8db6-4b5ba1467e1a
June 2010
Rodamporn, Somphop
43aa084a-2a90-41de-8db6-4b5ba1467e1a
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Brown, Andrew D.
5c19e523-65ec-499b-9e7c-91522017d7e0
Rodamporn, Somphop
(2010)
Microfluidic systems for cell transfection using sonoporation and electroporation.
University of Southampton, School of Electronics and Computer Science, Doctoral Thesis, 257pp.
Record type:
Thesis
(Doctoral)
Abstract
Studies into sonoporation and electroporation have grown rapidly in biotechnology and medicine in recent years. This research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation system and electroporation system with certain biological cells. Ultrasonic standing waves, previously used for ultrasonic particle manipulation, have been used in the development of the sonoporation aspects of the system. MATLAB has also been used to analyse the required acoustic conditions within the chamber. Furthermore, the electroporation system makes use of a relatively simple circuit consisting of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The electroporation system has been designed, developed and tested. The system was evaluated with HeLa cells, THP-1 cells and plasmid DNA (pEGFP-N1) to evaluate transfection rates under a variety of sonoporation conditions. This study also determined cell viability under a range of sonoporation and electroporation conditions.
Text
Thesis_s.rodamporn.pdf
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More information
Published date: June 2010
Organisations:
University of Southampton
Identifiers
Local EPrints ID: 159193
URI: http://eprints.soton.ac.uk/id/eprint/159193
PURE UUID: 52fc0474-3a3a-43de-b6c2-0784d332fd1a
Catalogue record
Date deposited: 16 Jul 2010 13:20
Last modified: 14 Mar 2024 02:37
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Contributors
Author:
Somphop Rodamporn
Thesis advisor:
Steve Beeby
Thesis advisor:
Andrew D. Brown
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