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The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea

Zeeuwen, Patrick L.J.M., van Vlijmen-Willems, Ivonne M.J.J., Cheng, Tsing, Rodijk-Olthuis, Diana, Hitomi, Kiyotaka, Hara-Nishimura, Ikuko, John, Susan, Smyth, Neil, Reinheckel, Thomas, Hendriks, Wiljan J.A.J. and Schalkwijk, Joost (2010) The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea The FASEB Journal (doi:10.1096/fj.10-155879).

Record type: Article


Cystatin M/E (CST6) is a nonredundant, epithelium-specific protease inhibitor with a presumed role in epidermal differentiation and tumor suppression. We have previously reported that cystatin M/E deficiency in Cst6(-)(/)(-) mice causes neonatal lethality because of excessive transepidermal water loss. Biochemical evidence suggests that cystatin M/E controls the activity of legumain, cathepsin L, cathepsin V, and transglutaminase-3. Using a genetic approach we sought to define the role of cystatin M/E in epithelial biology by identification of its target proteases and their downstream functions. Ablation of cathepsin L in a Cst6(-)(/)(-) background (Cst6(-)(/)(-)Ctsl(-)(/)(-) double-knockout mice) restored viability and resulted in normalization of stratum corneum morphology. Ablation of legumain or transglutaminase-3 in Cst6(-)(/)(-) mice, however, did not rescue the lethal phenotype. Intriguingly, both Cst6(-)(/)(-)Ctsl(-)(/)(-) and Cst6(-)(/)(-)Ctsl(+)(/)(-) mice were viable, but the absence of cystatin M/E caused scarring alopecia in adult animals. In the cornea of Cst6(-)(/)(-)Ctsl(+)(/)(-) mice, we observed keratitis, hyperplasia, and transition to a cornified epithelium. Evidence is provided that activation of cathepsin D and transglutaminase-1 are downstream events, dependent of cathepsin L activity. We conclude that a tightly regulated balance between cathepsin L and cystatin M/E is essential for tissue integrity in epidermis, hair follicles, and corneal epithelium.-Zeeuwen, P.L.J.M., van Vlijmen-Willems, I.M.J.J., Cheng, T., Rodijk-Olthuis, D., Hitomi, K., Hara-Nishimura, I., John, S., Smyth, N., Reinheckel, T., Hendriks, W.J.A.J., Schalkwijk, J. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles and cornea.

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Published date: 21 May 2010
Organisations: Biological Sciences


Local EPrints ID: 159373
ISSN: 0892-6638
PURE UUID: 6ecb6b16-09e4-4910-9bdf-7c3400413451

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Date deposited: 30 Jun 2010 08:29
Last modified: 18 Jul 2017 12:37

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Author: Patrick L.J.M. Zeeuwen
Author: Ivonne M.J.J. van Vlijmen-Willems
Author: Tsing Cheng
Author: Diana Rodijk-Olthuis
Author: Kiyotaka Hitomi
Author: Ikuko Hara-Nishimura
Author: Susan John
Author: Neil Smyth
Author: Thomas Reinheckel
Author: Wiljan J.A.J. Hendriks
Author: Joost Schalkwijk

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