Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human fallopian tubes in vitro
Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human fallopian tubes in vitro
In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase
(LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with
high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a
significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS.
Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor
TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release, which correlated with a ~50% reduction in gonococcal numbers.
Moreover, treatment of normal FT explants with an exogenous NO donor, SNAP, did not induce significant
cellular damage. Taken together, our data suggest that NO does not contribute to cellular damage during
infection of the human FT with Neisseria gonorrhoeae.
cellular damage, fallopian tubes, neisseria gonorrhoeae, nitric oxide
39-50
Garcia, Katherine
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Rubilar, Paulina S.
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Vargas, Macarena F.
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Cardenas, Hugo
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Rios, Miguel A.
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Orihuela, Pedro A.
61dbebbd-8a51-438f-82ad-db9c18b8e09d
Vargas, Renato H.
6102d5d4-b80a-4134-a9f7-53b8d48edcbc
Fuhrer, Juan
f9c79a5a-8eba-4143-941a-03240684a56e
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Velasquez, Luis A.
3a9da5d4-5091-4b16-9475-4e7e48f8d2f0
2010
Garcia, Katherine
5100c0c0-7313-45d5-a38d-539ef9d708d6
Rubilar, Paulina S.
11e23032-0ce8-4b05-acef-f6791843d8ee
Vargas, Macarena F.
20857707-a5e3-4c2f-853f-78932f66c5a0
Cardenas, Hugo
655f8130-647f-413a-8742-1b11d459e4c9
Rios, Miguel A.
0723b533-0c0a-478c-ab85-58ed3db31ba2
Orihuela, Pedro A.
61dbebbd-8a51-438f-82ad-db9c18b8e09d
Vargas, Renato H.
6102d5d4-b80a-4134-a9f7-53b8d48edcbc
Fuhrer, Juan
f9c79a5a-8eba-4143-941a-03240684a56e
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Velasquez, Luis A.
3a9da5d4-5091-4b16-9475-4e7e48f8d2f0
Garcia, Katherine, Rubilar, Paulina S., Vargas, Macarena F., Cardenas, Hugo, Rios, Miguel A., Orihuela, Pedro A., Vargas, Renato H., Fuhrer, Juan, Heckels, John E., Christodoulides, Myron and Velasquez, Luis A.
(2010)
Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human fallopian tubes in vitro.
Biological Research, 43, .
(doi:10.4067/S0716-97602010000100006).
Abstract
In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase
(LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with
high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a
significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS.
Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor
TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release, which correlated with a ~50% reduction in gonococcal numbers.
Moreover, treatment of normal FT explants with an exogenous NO donor, SNAP, did not induce significant
cellular damage. Taken together, our data suggest that NO does not contribute to cellular damage during
infection of the human FT with Neisseria gonorrhoeae.
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Published date: 2010
Keywords:
cellular damage, fallopian tubes, neisseria gonorrhoeae, nitric oxide
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Local EPrints ID: 160441
URI: http://eprints.soton.ac.uk/id/eprint/160441
ISSN: 0716-9760
PURE UUID: b52f627d-d80c-4019-836e-f587e2fa163a
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Date deposited: 14 Jul 2010 12:58
Last modified: 14 Mar 2024 02:34
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Contributors
Author:
Katherine Garcia
Author:
Paulina S. Rubilar
Author:
Macarena F. Vargas
Author:
Hugo Cardenas
Author:
Miguel A. Rios
Author:
Pedro A. Orihuela
Author:
Renato H. Vargas
Author:
Juan Fuhrer
Author:
Luis A. Velasquez
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