Identification of novel pneumolysin alleles from paediatric carriage isolates of Streptococcus pneumoniae
Identification of novel pneumolysin alleles from paediatric carriage isolates of Streptococcus pneumoniae
Pneumolysin (Ply) is a major virulence factor of Streptococcus pneumoniae and is produced by all known clinical isolates of pneumococci. Pneumolysin toxoids are being considered as vaccine candidates. We investigated the diversity of pneumolysin among 194 nasopharyngeal pneumococci characterized by serotyping and multilocus sequence typing (MLST). Eight Ply protein alleles were identified, four of which were novel. The 4 novel alleles varied at 10 different amino acid positions, from a total of 147, 3 of these substitutions have been previously reported in different combinations. The protein allele correlated closely with MLST. It is critical that the presence of pneumolysin variants is considered with regards to the potential use of Ply in future vaccine formulations, as variation in Ply amino acid sequence may influence the immunogenicity of vaccines based on the presence of an individual Ply allele.
The GenBank/EMBL/DDBJ accession numbers for the ply gene sequences of S. pneumoniae reported in this work are GU968217 to GU968411.
gdna, genomic dna, mlst, multilocus sequence typing, st, sequence type
808-814
Jefferies, J.M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Tocheva, A.S.
84454eb2-8171-43b2-923c-54d224c7cde1
Rubery, H.
3f76c976-03ff-4754-a516-9ba858a47c79
Bennett, J.
597dc7ed-0dfd-4b22-a670-b05549d5c08d
Garland, Joanna
7e673e59-abec-4f26-8689-c97edfe9ef22
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Faust, S.N.
f97df780-9f9b-418e-b349-7adf63e150c1
Smith, A.G.
8051e9d5-c35f-49c0-a2f0-91501f9aa72f
Mitchell, T.J.
927279e4-ea84-4ad0-aa32-314f0f3ae8e9
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
July 2010
Jefferies, J.M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Tocheva, A.S.
84454eb2-8171-43b2-923c-54d224c7cde1
Rubery, H.
3f76c976-03ff-4754-a516-9ba858a47c79
Bennett, J.
597dc7ed-0dfd-4b22-a670-b05549d5c08d
Garland, Joanna
7e673e59-abec-4f26-8689-c97edfe9ef22
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Faust, S.N.
f97df780-9f9b-418e-b349-7adf63e150c1
Smith, A.G.
8051e9d5-c35f-49c0-a2f0-91501f9aa72f
Mitchell, T.J.
927279e4-ea84-4ad0-aa32-314f0f3ae8e9
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Jefferies, J.M.C., Tocheva, A.S., Rubery, H., Bennett, J., Garland, Joanna, Christodoulides, Myron, Faust, S.N., Smith, A.G., Mitchell, T.J. and Clarke, S.C.
(2010)
Identification of novel pneumolysin alleles from paediatric carriage isolates of Streptococcus pneumoniae.
Journal of Medical Microbiology, 59 (7), .
(doi:10.1099/jmm.0.018663-0).
(PMID:20339017)
Abstract
Pneumolysin (Ply) is a major virulence factor of Streptococcus pneumoniae and is produced by all known clinical isolates of pneumococci. Pneumolysin toxoids are being considered as vaccine candidates. We investigated the diversity of pneumolysin among 194 nasopharyngeal pneumococci characterized by serotyping and multilocus sequence typing (MLST). Eight Ply protein alleles were identified, four of which were novel. The 4 novel alleles varied at 10 different amino acid positions, from a total of 147, 3 of these substitutions have been previously reported in different combinations. The protein allele correlated closely with MLST. It is critical that the presence of pneumolysin variants is considered with regards to the potential use of Ply in future vaccine formulations, as variation in Ply amino acid sequence may influence the immunogenicity of vaccines based on the presence of an individual Ply allele.
The GenBank/EMBL/DDBJ accession numbers for the ply gene sequences of S. pneumoniae reported in this work are GU968217 to GU968411.
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Published date: July 2010
Keywords:
gdna, genomic dna, mlst, multilocus sequence typing, st, sequence type
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Local EPrints ID: 162277
URI: http://eprints.soton.ac.uk/id/eprint/162277
ISSN: 0022-2615
PURE UUID: 5e9fc3c9-a945-4941-a7b4-c273e088f59b
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Date deposited: 17 Aug 2010 15:51
Last modified: 14 Mar 2024 02:51
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Author:
J.M.C. Jefferies
Author:
A.S. Tocheva
Author:
H. Rubery
Author:
J. Bennett
Author:
Joanna Garland
Author:
A.G. Smith
Author:
T.J. Mitchell
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