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Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders.

Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders.
Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders.
The European Myeloma Network (EMN) organized two flow cytometry workshops. The first aimed to identify specific indications for flow cytometry in patients with monoclonal gammopathies, and consensus technical approaches through a questionnaire-based review of current practice in participating laboratories. The second aimed to resolve outstanding technical issues and develop a consensus approach to analysis of plasma cells. The primary clinical applications identified were: differential diagnosis of neoplastic plasma cell disorders from reactive plasmacytosis; identifying risk of progression in patients with MGUS and detecting minimal residual disease. A range of technical recommendations were identified, including: 1) CD38, CD138 and CD45 should all be included in at least one tube for plasma cell identification and enumeration. The primary gate should be based on CD38 vs. CD138 expression; 2) after treatment, clonality assessment is only likely to be informative when combined with immunophenotype to detect abnormal cells. Flow cytometry is suitable for demonstrating a stringent complete remission; 3) for detection of abnormal plasma cells, a minimal panel should include CD19 and CD56. A preferred panel would also include CD20, CD117, CD28 and CD27; 4) discrepancies between the percentage of plasma cells detected by flow cytometry and morphology are primarily related to sample quality and it is, therefore, important to determine that marrow elements are present in follow-up samples, particularly normal plasma cells in MRD negative cases.
flow cytometry, myeloma, monoclonal gammopathies of undetermined significance
0390-6078
431-438
Rawstron, Andy C
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Orfao, Alberto
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Beksac, Meral
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Bezdickova, Ludmila
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Brooimans, Rik A
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Bumbea, Horia
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Dalva, Klara
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Fuhler, Gwenny
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Gratama, Jan
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Hose, Dirk
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Kovarova, Lucie
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Lioznov, Michael
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Mateo, Gema
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Morilla, Ricardo
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Mylin, Anne K
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Omedé, Paola
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Pellat-Deceunynck, Catherine
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Perez Andres, Martin
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Petrucci, Maria
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Ruggeri, Marina
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Rymkiewicz, Grzegorz
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Schmitz, Alexander
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Schreder, Martin
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Seynaeve, Carine
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Spacek, Martin
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de Tute, Ruth M
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Van Valckenborgh, Els
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Weston-Bell, Nicola J.
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Owen, Roger G
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San Miguel, Jesús F
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Sonneveld, Pieter
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Johnsen, Hans E
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Rawstron, Andy C
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Orfao, Alberto
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Beksac, Meral
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Bezdickova, Ludmila
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Brooimans, Rik A
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Bumbea, Horia
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Dalva, Klara
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Fuhler, Gwenny
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Gratama, Jan
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Hose, Dirk
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Kovarova, Lucie
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Lioznov, Michael
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Mateo, Gema
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Morilla, Ricardo
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Mylin, Anne K
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Omedé, Paola
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Pellat-Deceunynck, Catherine
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Perez Andres, Martin
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Petrucci, Maria
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Ruggeri, Marina
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Rymkiewicz, Grzegorz
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Schmitz, Alexander
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Schreder, Martin
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Seynaeve, Carine
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Spacek, Martin
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de Tute, Ruth M
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Van Valckenborgh, Els
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Weston-Bell, Nicola J.
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Owen, Roger G
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San Miguel, Jesús F
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Sonneveld, Pieter
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Johnsen, Hans E
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Rawstron, Andy C, Orfao, Alberto, Beksac, Meral, Bezdickova, Ludmila, Brooimans, Rik A, Bumbea, Horia, Dalva, Klara, Fuhler, Gwenny, Gratama, Jan, Hose, Dirk, Kovarova, Lucie, Lioznov, Michael, Mateo, Gema, Morilla, Ricardo, Mylin, Anne K, Omedé, Paola, Pellat-Deceunynck, Catherine, Perez Andres, Martin, Petrucci, Maria, Ruggeri, Marina, Rymkiewicz, Grzegorz, Schmitz, Alexander, Schreder, Martin, Seynaeve, Carine, Spacek, Martin, de Tute, Ruth M, Van Valckenborgh, Els, Weston-Bell, Nicola J., Owen, Roger G, San Miguel, Jesús F, Sonneveld, Pieter and Johnsen, Hans E (2008) Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders. Haematologica, 93 (3), 431-438. (doi:10.3324/haematol.11080).

Record type: Article

Abstract

The European Myeloma Network (EMN) organized two flow cytometry workshops. The first aimed to identify specific indications for flow cytometry in patients with monoclonal gammopathies, and consensus technical approaches through a questionnaire-based review of current practice in participating laboratories. The second aimed to resolve outstanding technical issues and develop a consensus approach to analysis of plasma cells. The primary clinical applications identified were: differential diagnosis of neoplastic plasma cell disorders from reactive plasmacytosis; identifying risk of progression in patients with MGUS and detecting minimal residual disease. A range of technical recommendations were identified, including: 1) CD38, CD138 and CD45 should all be included in at least one tube for plasma cell identification and enumeration. The primary gate should be based on CD38 vs. CD138 expression; 2) after treatment, clonality assessment is only likely to be informative when combined with immunophenotype to detect abnormal cells. Flow cytometry is suitable for demonstrating a stringent complete remission; 3) for detection of abnormal plasma cells, a minimal panel should include CD19 and CD56. A preferred panel would also include CD20, CD117, CD28 and CD27; 4) discrepancies between the percentage of plasma cells detected by flow cytometry and morphology are primarily related to sample quality and it is, therefore, important to determine that marrow elements are present in follow-up samples, particularly normal plasma cells in MRD negative cases.

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More information

Published date: March 2008
Keywords: flow cytometry, myeloma, monoclonal gammopathies of undetermined significance

Identifiers

Local EPrints ID: 162367
URI: http://eprints.soton.ac.uk/id/eprint/162367
ISSN: 0390-6078
PURE UUID: c024dbc1-1d10-48ad-bdbb-fdfa188a1abb
ORCID for Nicola J. Weston-Bell: ORCID iD orcid.org/0000-0003-0075-7276

Catalogue record

Date deposited: 20 Aug 2010 08:12
Last modified: 14 Mar 2024 02:02

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Contributors

Author: Andy C Rawstron
Author: Alberto Orfao
Author: Meral Beksac
Author: Ludmila Bezdickova
Author: Rik A Brooimans
Author: Horia Bumbea
Author: Klara Dalva
Author: Gwenny Fuhler
Author: Jan Gratama
Author: Dirk Hose
Author: Lucie Kovarova
Author: Michael Lioznov
Author: Gema Mateo
Author: Ricardo Morilla
Author: Anne K Mylin
Author: Paola Omedé
Author: Catherine Pellat-Deceunynck
Author: Martin Perez Andres
Author: Maria Petrucci
Author: Marina Ruggeri
Author: Grzegorz Rymkiewicz
Author: Alexander Schmitz
Author: Martin Schreder
Author: Carine Seynaeve
Author: Martin Spacek
Author: Ruth M de Tute
Author: Els Van Valckenborgh
Author: Nicola J. Weston-Bell ORCID iD
Author: Roger G Owen
Author: Jesús F San Miguel
Author: Pieter Sonneveld
Author: Hans E Johnsen

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