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Establishment of the first World Health Organization international genetic reference panel for quantitation of BCR-ABL mRNA

White, Helen E., Matejtschuk, Paul, Rigsby, Peter, Gabert, Jean, Lin, Feng, Wang, Y. Lynn, Branford, Susan, Muller, Martin C., Beaufils, Nathalie, Beillard, Emmanuel, Colomer, Dolors, Dvorakova, Dana, Ehrencrona, Hans, Goh, Hyun-Gyung, El Housni, Hakim, Jones, Dan, Kairisto, Veli, Kamel-Reid, Suzanne, Kim, Dong-Wook, Langabeer, Stephen, Ma, Edmund S.K., Press, Richard D., Romeo, Giuliana, Wang, Lihui, Zoi, Katerina, Hughes, Timothy, Saglio, Giuseppe, Hochhaus, Andreas, Goldman, John M., Metcalfe, Paul and Cross, Nicholas C.P. (2010) Establishment of the first World Health Organization international genetic reference panel for quantitation of BCR-ABL mRNA Blood, 116, (22), pp. 111-117. (doi:10.1182/blood-2010-06-291641). (PMID:20720184).

Record type: Article


Serial quantitation of BCR-ABL mRNA levels is an important indicator of therapeutic response for patients with chronic myelogenous leukemia and Philadelphia-chromosome positive acute lymphoblastic leukemia, but there is substantial variation in the real time quantitative PCR (RQ-PCR) methodologies that are employed by different testing laboratories. To help improve the comparability of results between centers we sought to develop accredited reference reagents that are directly linked to the BCR-ABL international scale. Following assessment of candidate cell lines, a reference material panel comprising four different dilution levels of freeze dried preparations of K562 cells diluted in HL60 cells was prepared. Following performance evaluation, the materials were assigned fixed % BCR-ABL/control gene values according to the International Scale. A recommendation that the four materials be established as the 1st World Health Organization (WHO) International Genetic Reference Panel for quantitation of BCR-ABL translocation by RQ-PCR was approved by the Expert Committee on Biological Standardization of the WHO in November 2009. We consider that the development of these reagents is a significant milestone in the standardization of this clinically important test, but since they are a limited resource we suggest that their availability is restricted to manufacturers of secondary reference materials.

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e-pub ahead of print date: 18 August 2010
Published date: 25 November 2010
Organisations: Human Genetics


Local EPrints ID: 162459
ISSN: 0006-4971
PURE UUID: da04a8cd-b24b-411d-8ba7-38ac06a28255
ORCID for Nicholas C.P. Cross: ORCID iD

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Date deposited: 23 Aug 2010 07:55
Last modified: 18 Jul 2017 12:32

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Author: Helen E. White
Author: Paul Matejtschuk
Author: Peter Rigsby
Author: Jean Gabert
Author: Feng Lin
Author: Y. Lynn Wang
Author: Susan Branford
Author: Martin C. Muller
Author: Nathalie Beaufils
Author: Emmanuel Beillard
Author: Dolors Colomer
Author: Dana Dvorakova
Author: Hans Ehrencrona
Author: Hyun-Gyung Goh
Author: Hakim El Housni
Author: Dan Jones
Author: Veli Kairisto
Author: Suzanne Kamel-Reid
Author: Dong-Wook Kim
Author: Stephen Langabeer
Author: Edmund S.K. Ma
Author: Richard D. Press
Author: Giuliana Romeo
Author: Lihui Wang
Author: Katerina Zoi
Author: Timothy Hughes
Author: Giuseppe Saglio
Author: Andreas Hochhaus
Author: John M. Goldman
Author: Paul Metcalfe

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