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In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets

In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets
In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets
Neurogenin 3 (NGN3) commits pancreatic progenitors to an islet cell fate. We induced NGN3 expression and identified up-regulation of the gene encoding the small molecular mass GTP-binding protein, RAB3B. RAB3B localized to the cytoplasm of human beta-cells, both during the fetal period and post-natally. Genes encoding alternative RAB3 proteins and RAB27A were unaltered by NGN3 expression and in human adult islets their transcripts were many fold less prevalent than those of RAB3B. The regulation of insulin exocytosis in rodent beta-cells and responsiveness to incretins is reliant upon Rab family members, notably Rab3a and Rab27a, but not Rab3b. Our results support an important inter-species difference in regulating insulin exocytosis where RAB3B is the most expressed isoform in human beta-cells
151-161
Piper Hanley, Karen
f11a1076-ad7d-446e-bd59-96814475e06f
Hearn, Tom
2665cc10-6632-47cb-9460-bd0ea745380e
Berry, Andrew
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Carvell, Melanie
548fb0c8-53e5-471d-8376-baf4bfc38210
Patch, Ann-Marie
eec11d53-c5de-4107-a1de-1175f7ab9583
Williams, Louise
e23f49ce-5ff1-4e7f-b377-aa90ddc3b393
Sugden, Sarah
e7b9288f-4b2b-4f72-976c-e5bc53e91d83
Wilson, David I.
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Ellard, Sian
6c9b0ede-8980-4602-b063-444b165baa09
Hanley, Neil A.
0cbe18e0-6273-4d44-b897-0ce031196009
Piper Hanley, Karen
f11a1076-ad7d-446e-bd59-96814475e06f
Hearn, Tom
2665cc10-6632-47cb-9460-bd0ea745380e
Berry, Andrew
29f51eb1-fe40-4d02-bb19-9d0968a6aa00
Carvell, Melanie
548fb0c8-53e5-471d-8376-baf4bfc38210
Patch, Ann-Marie
eec11d53-c5de-4107-a1de-1175f7ab9583
Williams, Louise
e23f49ce-5ff1-4e7f-b377-aa90ddc3b393
Sugden, Sarah
e7b9288f-4b2b-4f72-976c-e5bc53e91d83
Wilson, David I.
1500fca1-7082-4271-95f4-691f1d1252a2
Ellard, Sian
6c9b0ede-8980-4602-b063-444b165baa09
Hanley, Neil A.
0cbe18e0-6273-4d44-b897-0ce031196009

Piper Hanley, Karen, Hearn, Tom, Berry, Andrew, Carvell, Melanie, Patch, Ann-Marie, Williams, Louise, Sugden, Sarah, Wilson, David I., Ellard, Sian and Hanley, Neil A. (2010) In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets. Journal of Endocrinology, 207 (2), 151-161. (doi:10.1677/JOE-10-0120).

Record type: Article

Abstract

Neurogenin 3 (NGN3) commits pancreatic progenitors to an islet cell fate. We induced NGN3 expression and identified up-regulation of the gene encoding the small molecular mass GTP-binding protein, RAB3B. RAB3B localized to the cytoplasm of human beta-cells, both during the fetal period and post-natally. Genes encoding alternative RAB3 proteins and RAB27A were unaltered by NGN3 expression and in human adult islets their transcripts were many fold less prevalent than those of RAB3B. The regulation of insulin exocytosis in rodent beta-cells and responsiveness to incretins is reliant upon Rab family members, notably Rab3a and Rab27a, but not Rab3b. Our results support an important inter-species difference in regulating insulin exocytosis where RAB3B is the most expressed isoform in human beta-cells

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Published date: November 2010

Identifiers

Local EPrints ID: 163129
URI: http://eprints.soton.ac.uk/id/eprint/163129
PURE UUID: adb0b432-776d-4eab-bc33-33ac605494ef

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Date deposited: 10 Sep 2010 13:28
Last modified: 14 Mar 2024 02:04

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Contributors

Author: Karen Piper Hanley
Author: Tom Hearn
Author: Andrew Berry
Author: Melanie Carvell
Author: Ann-Marie Patch
Author: Louise Williams
Author: Sarah Sugden
Author: David I. Wilson
Author: Sian Ellard
Author: Neil A. Hanley

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