The University of Southampton
University of Southampton Institutional Repository

A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture

Underwood, Timothy J., Derouet, Mathieu F., White, Michael J., Noble, Fergus, Moutasim, Karwan A., Smith, Eric, Drew, Paul A., Thomas, Gareth J., Primrose, John N. and Blaydes, Jeremy P. (2010) A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture Biology of the Cell, 102, pp. 635-644. (doi:10.1042/BC20100071). (PMID:20843300).

Record type: Article

Abstract

Background Information: Carcinoma of the oesophagus is the 6th leading cause of cancer death in the western world and is associated with a 5-year survival of less than 15%. Recent evidence suggests that stromal-epithelial interactions are fundamental in carcinogenesis. The advent of co-culture techniques permits the investigation of cross-talk between the stroma and epithelium in a physiological setting. We have characterised a histologically representative oesophageal organotypic model, and used it to compare the most commonly used squamous oesophageal cell line, HET-1A, with primary oesophageal squamous cells for use in studies of the oesophageal epithelium in vitro.

Results: When grown in an organotypic culture with normal fibroblasts the oesophageal carcinoma cell lines OE21 (squamous) and OE19 (adenocarcinoma) morphologically resembled the tumour of origin with evidence of stromal invasion and mucus production respectively. However, HET-1A cells, which were derived from normal squamous oesophageal cells, appeared dysplastic and failed to display evidence of squamous differentiation. By comparison with primary oesophageal epithelial cells the HET-1A cells were highly proliferative and did not express the epithelial markers E-cadherin or CK5/6, or the stratified epithelial marker ?Np63, but did express the mesenchymal markers vimentin and N-cadherin.

Conclusion: Studies of epithelial carcinogenesis will benefit from culture systems which allow the manipulation of the stromal and epithelial layers independently. We have developed an organotypic culture using primary oesophageal squamous cells and fibroblasts in which a stratified epithelium with a proliferative basal layer that stains strongly for ?Np63 develops. This model will be suitable for the study of the molecular events in the development of Barrett's oesophagus. The most commonly used normal oesophageal squamous cell line, HET-1A, does not have the characteristics of normal oesophageal squamous cells, and should not be used in models of the normal oesophageal epithelium. Until more representative cell lines are available, future studies in oesophageal cancer will be reliant on the availability and manipulation of primary tissue.

Full text not available from this repository.

More information

Published date: 15 September 2010
Keywords: het-1A, oesophageal carcinoma, organotypic model, p63

Identifiers

Local EPrints ID: 164155
URI: http://eprints.soton.ac.uk/id/eprint/164155
ISSN: 0248-4900
PURE UUID: 04d5d130-1429-4e39-b430-b7b88bd9b441
ORCID for Timothy J. Underwood: ORCID iD orcid.org/0000-0001-9455-2188
ORCID for John N. Primrose: ORCID iD orcid.org/0000-0002-2069-7605

Catalogue record

Date deposited: 23 Dec 2010 11:41
Last modified: 18 Jul 2017 12:28

Export record

Altmetrics

Contributors

Author: Mathieu F. Derouet
Author: Michael J. White
Author: Fergus Noble
Author: Eric Smith
Author: Paul A. Drew

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×