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Honey promotes angiogenic activity in the rat aortic ring assay

Honey promotes angiogenic activity in the rat aortic ring assay
Honey promotes angiogenic activity in the rat aortic ring assay
Objective: To investigate possible effects of honey on angiogenesis, using in vitro analogues of angiogenesis and an endothelial proliferation assay.

Method: Using an in vitro rat aortic ring assay we compared pseudotubule formation by medicinal honey (Activon), supermarket honey (Rowse) and a honey-based ointment (Mesitran), with that of artificial honey (70% w/w sugar glucose/fructose). Pseudotubules were analysed using TCS Cellworks AngioSys software. The Angiokit system was used to validate the results. Using the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium. Bromide] assay, toxicity was also assessed on human umbilical vein endothelial cells (HUVEC) directly adherent to plastic.

Results: All honey preparations stimulated pseudotubule formation, maximal at around 0.2% honey. Medicinal honeys were more active than Rowse. The effect was not attributable to the sugar content. Among the honeys tested, the Manuka-based Activon preparation reduced residual viable biomass compared with a sugar control at >0.32% v/v concentration. Rowse had a similar effect only at 2.5%, the highest dose tested.

Conclusion: The influence of honey constituents on angiogenesis in a wound dressing context is likely to be positive, but would depend on the effective dilution of the honey and the penetration of the active constituents against an osmotic gradient. The extent to which this occurs has yet to be established.

Conflict of interest: This work was conceived, designed and executed by the authors. Medical honey preparations were supplied unconditionally but free of charge by the distributors.
0969-0700
440-446
Rossiter, K.
95737c7e-0829-4a00-a3e4-3089d65a3dca
Cooper, A.J.
8a21c297-eda3-4479-8e81-1de258c8e2a1
Voegeli, D.
e6f5d112-55b0-40c1-a6ad-8929a2d84a10
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Rossiter, K.
95737c7e-0829-4a00-a3e4-3089d65a3dca
Cooper, A.J.
8a21c297-eda3-4479-8e81-1de258c8e2a1
Voegeli, D.
e6f5d112-55b0-40c1-a6ad-8929a2d84a10
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21

Rossiter, K., Cooper, A.J., Voegeli, D. and Lwaleed, B.A. (2010) Honey promotes angiogenic activity in the rat aortic ring assay. Journal of Wound Care, 19 (10), 440-446.

Record type: Article

Abstract

Objective: To investigate possible effects of honey on angiogenesis, using in vitro analogues of angiogenesis and an endothelial proliferation assay.

Method: Using an in vitro rat aortic ring assay we compared pseudotubule formation by medicinal honey (Activon), supermarket honey (Rowse) and a honey-based ointment (Mesitran), with that of artificial honey (70% w/w sugar glucose/fructose). Pseudotubules were analysed using TCS Cellworks AngioSys software. The Angiokit system was used to validate the results. Using the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium. Bromide] assay, toxicity was also assessed on human umbilical vein endothelial cells (HUVEC) directly adherent to plastic.

Results: All honey preparations stimulated pseudotubule formation, maximal at around 0.2% honey. Medicinal honeys were more active than Rowse. The effect was not attributable to the sugar content. Among the honeys tested, the Manuka-based Activon preparation reduced residual viable biomass compared with a sugar control at >0.32% v/v concentration. Rowse had a similar effect only at 2.5%, the highest dose tested.

Conclusion: The influence of honey constituents on angiogenesis in a wound dressing context is likely to be positive, but would depend on the effective dilution of the honey and the penetration of the active constituents against an osmotic gradient. The extent to which this occurs has yet to be established.

Conflict of interest: This work was conceived, designed and executed by the authors. Medical honey preparations were supplied unconditionally but free of charge by the distributors.

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Published date: 14 October 2010

Identifiers

Local EPrints ID: 165675
URI: http://eprints.soton.ac.uk/id/eprint/165675
ISSN: 0969-0700
PURE UUID: 0618c0f9-c597-445a-8197-54cd832559cd
ORCID for D. Voegeli: ORCID iD orcid.org/0000-0003-3457-7177
ORCID for B.A. Lwaleed: ORCID iD orcid.org/0000-0001-5748-4892

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Date deposited: 19 Oct 2010 08:31
Last modified: 06 Aug 2024 01:39

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Contributors

Author: K. Rossiter
Author: A.J. Cooper
Author: D. Voegeli ORCID iD
Author: B.A. Lwaleed ORCID iD

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