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Time dependence of cyclic tensile strain on collagen production in tendon fascicles

Time dependence of cyclic tensile strain on collagen production in tendon fascicles
Time dependence of cyclic tensile strain on collagen production in tendon fascicles
Mechanical loading is a regulator of tissue metabolism in tendon, which may lead to alterations in structural and mechanical properties via mechanotransduction processes. The present study investigated specified responses of tenocyte metabolism in isolated tendon fascicles subjected to four loading regimes. Cyclic tensile strain of 3% amplitude superimposed on a 2% static strain was applied to the fascicles for 10 min, 1, 6 or 24 h of a 24-h incubation period. Collagen synthesis, assessed by [3H]-proline incorporation, was upregulated by the 24 h straining regime, but was inhibited by the 10-min regime. Cyclic strain enhanced the retention of newly synthesised collagen within the matrix. More than 90% of the newly synthesised collagen was retained in all cases, but the long-term application of cyclic strain had less pronounced effects on the retention. These results indicate that collagen synthesis by tenocytes is controlled by a complex mechanosensitive process with a temporal component
0006-291X
399-404
Maeda, Eijiro
15867696-0c06-4c07-a637-fc8c603695d7
Shelton, Julia C.
04ee5976-0824-48a8-ae1d-b0e147d8a879
Bader, Dan L.
06079726-5aa3-49cd-ad71-402ab4cd3255
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Maeda, Eijiro
15867696-0c06-4c07-a637-fc8c603695d7
Shelton, Julia C.
04ee5976-0824-48a8-ae1d-b0e147d8a879
Bader, Dan L.
06079726-5aa3-49cd-ad71-402ab4cd3255
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871

Maeda, Eijiro, Shelton, Julia C., Bader, Dan L. and Lee, David A. (2007) Time dependence of cyclic tensile strain on collagen production in tendon fascicles. Biochemical and Biophysical Research Communications, 362 (2), 399-404. (doi:10.1016/j.bbrc.2007.08.029). (PMID:17719009)

Record type: Article

Abstract

Mechanical loading is a regulator of tissue metabolism in tendon, which may lead to alterations in structural and mechanical properties via mechanotransduction processes. The present study investigated specified responses of tenocyte metabolism in isolated tendon fascicles subjected to four loading regimes. Cyclic tensile strain of 3% amplitude superimposed on a 2% static strain was applied to the fascicles for 10 min, 1, 6 or 24 h of a 24-h incubation period. Collagen synthesis, assessed by [3H]-proline incorporation, was upregulated by the 24 h straining regime, but was inhibited by the 10-min regime. Cyclic strain enhanced the retention of newly synthesised collagen within the matrix. More than 90% of the newly synthesised collagen was retained in all cases, but the long-term application of cyclic strain had less pronounced effects on the retention. These results indicate that collagen synthesis by tenocytes is controlled by a complex mechanosensitive process with a temporal component

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Published date: October 2007

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Local EPrints ID: 168969
URI: http://eprints.soton.ac.uk/id/eprint/168969
ISSN: 0006-291X
PURE UUID: bcbcc7d2-3d92-464d-803a-9508c9ad5889

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Date deposited: 08 Dec 2010 09:31
Last modified: 14 Mar 2024 02:18

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Contributors

Author: Eijiro Maeda
Author: Julia C. Shelton
Author: Dan L. Bader
Author: David A. Lee

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