The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs
The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs
Endogenous electrical activity has been detected in articular cartilage. It has previously been suggested that the associated electrical currents and potentials are important to the mechanotransduction processes in cartilage. The present study investigates the effects of direct current on cell proliferation and matrix synthesis, using the well established 3D chondrocyte – agarose model system.
Bovine chondrocytes isolated from metacarpalphalangeal joints were seeded in agarose constructs and exposed to a current density of 4 mA/cm2 for 6 h, a magnitude and period which was shown to maintain cell viability. The influence of the optimized electric stimulus was assessed by protein incorporation and mRNA measurements, using radiolabels and real-time QPCR, respectively.
Results indicated no systematic influences of electrical current on protein synthesis, cell proliferation and mRNA expression levels. These data suggest that both the mode of stimulation and the model system are critical for the in vitro modulation of chondrocyte metabolism
electric stimulation, chondrocyte, proteoglycan, collagen
229-243
Akanji, O.O.
49ec1295-ca48-4e46-bc0d-fe9d3efd5e14
Lee, D.A.
fbbf7169-d08b-4deb-ae87-a2cbd97c58e7
Bader, D.A.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
September 2008
Akanji, O.O.
49ec1295-ca48-4e46-bc0d-fe9d3efd5e14
Lee, D.A.
fbbf7169-d08b-4deb-ae87-a2cbd97c58e7
Bader, D.A.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Akanji, O.O., Lee, D.A. and Bader, D.A.
(2008)
The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs.
Journal of Biorheology, 45 (3-4), .
(doi:10.3233/BIR-2008-0473).
Abstract
Endogenous electrical activity has been detected in articular cartilage. It has previously been suggested that the associated electrical currents and potentials are important to the mechanotransduction processes in cartilage. The present study investigates the effects of direct current on cell proliferation and matrix synthesis, using the well established 3D chondrocyte – agarose model system.
Bovine chondrocytes isolated from metacarpalphalangeal joints were seeded in agarose constructs and exposed to a current density of 4 mA/cm2 for 6 h, a magnitude and period which was shown to maintain cell viability. The influence of the optimized electric stimulus was assessed by protein incorporation and mRNA measurements, using radiolabels and real-time QPCR, respectively.
Results indicated no systematic influences of electrical current on protein synthesis, cell proliferation and mRNA expression levels. These data suggest that both the mode of stimulation and the model system are critical for the in vitro modulation of chondrocyte metabolism
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Published date: September 2008
Additional Information:
Selected papers of the 5th International Symposium on Mechanobiology of Cartilage and Chondrocyte, Athens, May 2007
Keywords:
electric stimulation, chondrocyte, proteoglycan, collagen
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Local EPrints ID: 169029
URI: http://eprints.soton.ac.uk/id/eprint/169029
ISSN: 1867-0466
PURE UUID: ef211fb7-3d2c-4f2b-ba79-28c4f21baa9c
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Date deposited: 08 Dec 2010 15:10
Last modified: 14 Mar 2024 02:19
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Author:
O.O. Akanji
Author:
D.A. Lee
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