The University of Southampton
University of Southampton Institutional Repository

The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs

Akanji, O.O., Lee, D.A. and Bader, D.L. (2008) The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs Biorheology, 45, (3-4), pp. 229-243. (doi:10.3233/BIR-2008-0473).

Record type: Article


Endogenous electrical activity has been detected in articular cartilage. It has previously been suggested that the associated electrical currents and potentials are important to the mechanotransduction processes in cartilage. The present study investigates the effects of direct current on cell proliferation and matrix synthesis, using the well established 3D chondrocyte – agarose model system.

Bovine chondrocytes isolated from metacarpalphalangeal joints were seeded in agarose constructs and exposed to a current density of 4 mA/cm2 for 6 h, a magnitude and period which was shown to maintain cell viability. The influence of the optimized electric stimulus was assessed by protein incorporation and mRNA measurements, using radiolabels and real-time QPCR, respectively.

Results indicated no systematic influences of electrical current on protein synthesis, cell proliferation and mRNA expression levels. These data suggest that both the mode of stimulation and the model system are critical for the in vitro modulation of chondrocyte metabolism

Full text not available from this repository.

More information

Published date: 2008
Additional Information: Selected papers of the 5th International Symposium on Mechanobiology of Cartilage and Chondrocyte, Athens, May 2007
Keywords: electric stimulation, chondrocyte, proteoglycan, collagen


Local EPrints ID: 169029
PURE UUID: ef211fb7-3d2c-4f2b-ba79-28c4f21baa9c

Catalogue record

Date deposited: 08 Dec 2010 15:10
Last modified: 18 Jul 2017 12:19

Export record



Author: O.O. Akanji
Author: D.A. Lee
Author: D.L. Bader

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.