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The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs

Record type: Article

Endogenous electrical activity has been detected in articular cartilage. It has previously been suggested that the associated electrical currents and potentials are important to the mechanotransduction processes in cartilage. The present study investigates the effects of direct current on cell proliferation and matrix synthesis, using the well established 3D chondrocyte – agarose model system.

Bovine chondrocytes isolated from metacarpalphalangeal joints were seeded in agarose constructs and exposed to a current density of 4 mA/cm2 for 6 h, a magnitude and period which was shown to maintain cell viability. The influence of the optimized electric stimulus was assessed by protein incorporation and mRNA measurements, using radiolabels and real-time QPCR, respectively.

Results indicated no systematic influences of electrical current on protein synthesis, cell proliferation and mRNA expression levels. These data suggest that both the mode of stimulation and the model system are critical for the in vitro modulation of chondrocyte metabolism

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Citation

Akanji, O.O., Lee, D.A. and Bader, D.L. (2008) The effects of direct current stimulation on isolated chondrocytes seeded in 3D agarose constructs Biorheology, 45, (3-4), pp. 229-243. (doi:10.3233/BIR-2008-0473).

More information

Published date: 2008
Additional Information: Selected papers of the 5th International Symposium on Mechanobiology of Cartilage and Chondrocyte, Athens, May 2007
Keywords: electric stimulation, chondrocyte, proteoglycan, collagen

Identifiers

Local EPrints ID: 169029
URI: http://eprints.soton.ac.uk/id/eprint/169029
PURE UUID: ef211fb7-3d2c-4f2b-ba79-28c4f21baa9c

Catalogue record

Date deposited: 08 Dec 2010 15:10
Last modified: 18 Jul 2017 12:19

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Contributors

Author: O.O. Akanji
Author: D.A. Lee
Author: D.L. Bader

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