Biomechanical analysis of structural deformation in living cells
Biomechanical analysis of structural deformation in living cells
Most tissues are subject to some form of physiological mechanical loading which results in deformation of the cells triggering intracellular mechanotransduction pathways. This response to loading is generally essential for the health of the tissue, although more pronounced deformation may result in cell and tissue damage. In order to determine the biological response of cells to loading it is necessary to understand how cells and intracellular structures deform. This paper reviews the various loading systems that have been adopted for studying cell deformation both in situ within tissue explants and in isolated cell culture systems. In particular it describes loading systems which facilitate visualisation and subsequent quantification of cell deformation. The review also describes the associated microscopy and image analysis techniques. The review focuses on deformation of chondrocytes with additional information on a variety of other cell types including neurons, red blood cells, epithelial cells and skin and muscle cells
cell deformation, mechanotransduction, mechanics, confocal, biomechanics
951-963
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Knight, M.
57a45154-c5e4-40ea-8187-031cc55f94cd
26 August 2008
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Knight, M.
57a45154-c5e4-40ea-8187-031cc55f94cd
Abstract
Most tissues are subject to some form of physiological mechanical loading which results in deformation of the cells triggering intracellular mechanotransduction pathways. This response to loading is generally essential for the health of the tissue, although more pronounced deformation may result in cell and tissue damage. In order to determine the biological response of cells to loading it is necessary to understand how cells and intracellular structures deform. This paper reviews the various loading systems that have been adopted for studying cell deformation both in situ within tissue explants and in isolated cell culture systems. In particular it describes loading systems which facilitate visualisation and subsequent quantification of cell deformation. The review also describes the associated microscopy and image analysis techniques. The review focuses on deformation of chondrocytes with additional information on a variety of other cell types including neurons, red blood cells, epithelial cells and skin and muscle cells
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bader_and_knight_2008.pdf
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Published date: 26 August 2008
Keywords:
cell deformation, mechanotransduction, mechanics, confocal, biomechanics
Organisations:
Medicine
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Local EPrints ID: 169053
URI: http://eprints.soton.ac.uk/id/eprint/169053
ISSN: 0140-0118
PURE UUID: 2bb2aeee-363e-4f24-a801-2d26d5bbd504
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Date deposited: 09 Dec 2010 09:27
Last modified: 14 Mar 2024 02:19
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Author:
M. Knight
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