The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport properties
The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport properties
Limitations to nutrient transport provide a challenge to the development of 3D tissue-engineered constructs. A heterogeneous distribution of viable cells and functional matrix within the developing tissue is a common consequence. In the present study, a bioreactor was developed to perfuse fluid through cylindrical agarose constructs. The transport and distribution of dextran molecules (FD-4, FD-500, FD-2000) within the agarose was visualized in order to determine the bioreactors effectiveness for transport enhancement. By 24 h, the perfusion bioreactor achieved 529%, 395% and 294% higher concentrations of FD-4, FD-500 and FD-2000, respectively, than those solely due to diffusion. Of particular interest was the effectiveness of the bioreactor to transport molecules to the central region of the constructs. In this respect, the perfusion bioreactor was found to increase transportation of FD-4, FD-500 and FD-2000 by 30%, 291% and 222% over that of diffusion. Articular chondrocytes were cultured and perfused using the bioreactor. The improved molecular transport achieved led to an average 75% and 1340% increase of DNA and sulphated GAG, respectively at 20 days. More significantly was the 106% and 1603% increase of DNA and GAG, respectively, achieved at the central core of the 3D constructs
bioreactors, tissue engineering, molecular transport, fluid flow, perfusion chondrocytes
417-437
Eniwumide, Joshua O.
03fd5eca-734b-4732-ab77-edf1fa5b6a97
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
25 November 2009
Eniwumide, Joshua O.
03fd5eca-734b-4732-ab77-edf1fa5b6a97
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Eniwumide, Joshua O., Lee, David A. and Bader, Dan L.
(2009)
The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport properties.
Journal of Biorheology, 46 (5), .
(doi:10.3233/BIR-2009-0552).
(PMID:19940357)
Abstract
Limitations to nutrient transport provide a challenge to the development of 3D tissue-engineered constructs. A heterogeneous distribution of viable cells and functional matrix within the developing tissue is a common consequence. In the present study, a bioreactor was developed to perfuse fluid through cylindrical agarose constructs. The transport and distribution of dextran molecules (FD-4, FD-500, FD-2000) within the agarose was visualized in order to determine the bioreactors effectiveness for transport enhancement. By 24 h, the perfusion bioreactor achieved 529%, 395% and 294% higher concentrations of FD-4, FD-500 and FD-2000, respectively, than those solely due to diffusion. Of particular interest was the effectiveness of the bioreactor to transport molecules to the central region of the constructs. In this respect, the perfusion bioreactor was found to increase transportation of FD-4, FD-500 and FD-2000 by 30%, 291% and 222% over that of diffusion. Articular chondrocytes were cultured and perfused using the bioreactor. The improved molecular transport achieved led to an average 75% and 1340% increase of DNA and sulphated GAG, respectively at 20 days. More significantly was the 106% and 1603% increase of DNA and GAG, respectively, achieved at the central core of the 3D constructs
Text
eniwumide_2009.pdf
- Version of Record
More information
Published date: 25 November 2009
Keywords:
bioreactors, tissue engineering, molecular transport, fluid flow, perfusion chondrocytes
Identifiers
Local EPrints ID: 169363
URI: http://eprints.soton.ac.uk/id/eprint/169363
ISSN: 1867-0466
PURE UUID: 86846832-7260-4bc6-abcc-a9e438c9668d
Catalogue record
Date deposited: 14 Dec 2010 10:05
Last modified: 14 Mar 2024 02:20
Export record
Altmetrics
Contributors
Author:
Joshua O. Eniwumide
Author:
David A. Lee
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics