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Effect of intermittent cyclic tensile strain on collagen synthesis by tenocytes in isolated fascicles

Effect of intermittent cyclic tensile strain on collagen synthesis by tenocytes in isolated fascicles
Effect of intermittent cyclic tensile strain on collagen synthesis by tenocytes in isolated fascicles
Intermittent pattern of mechanical stimulation has been demonstrated to possess different regulatory effects on cell metabolism in many connective tissues, but little is known about tenocyte responses. A previous study has shown that the application of a small number of continuous cyclic strain inhibited collagen synthesis by tenocytes in explants, whereas a large number of strain cycles upregulated the synthesis. Thus, the present study tested the hypothesis that collagen synthesis is influenced by cyclic tensile strain provided in an intermittent manner. A total of 43,200 cycles of tensile strain, with a 3% amplitude superimposed on a 2% static strain was provided in four different intermittent patterns with different strain/unstrain periods: 10 minutes, 1 hour, 6 hours, and 12 hours. The amount of newly synthesised collagen, both those retained in strained fascicles and released into culture media, were not significantly altered by the application of different patters of intermittent cyclic strain. The present findings may suggest that, unlike other connective tissue cells, tenocyte responses are predominantly regulated by the total number of strain cycles.

1880-9863
510-517
Maeda, Eijiro
15867696-0c06-4c07-a637-fc8c603695d7
Shelton, Julia C.
04ee5976-0824-48a8-ae1d-b0e147d8a879
Bader, Dan L.
06079726-5aa3-49cd-ad71-402ab4cd3255
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Maeda, Eijiro
15867696-0c06-4c07-a637-fc8c603695d7
Shelton, Julia C.
04ee5976-0824-48a8-ae1d-b0e147d8a879
Bader, Dan L.
06079726-5aa3-49cd-ad71-402ab4cd3255
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871

Maeda, Eijiro, Shelton, Julia C., Bader, Dan L. and Lee, David A. (2009) Effect of intermittent cyclic tensile strain on collagen synthesis by tenocytes in isolated fascicles. [in special issue: Special Issue on Mechanical Behavior of Soft Tissues and their Substitutes] Journal of Biomechanical Science and Engineering, 4 (4), supplement SI, 510-517.

Record type: Article

Abstract

Intermittent pattern of mechanical stimulation has been demonstrated to possess different regulatory effects on cell metabolism in many connective tissues, but little is known about tenocyte responses. A previous study has shown that the application of a small number of continuous cyclic strain inhibited collagen synthesis by tenocytes in explants, whereas a large number of strain cycles upregulated the synthesis. Thus, the present study tested the hypothesis that collagen synthesis is influenced by cyclic tensile strain provided in an intermittent manner. A total of 43,200 cycles of tensile strain, with a 3% amplitude superimposed on a 2% static strain was provided in four different intermittent patterns with different strain/unstrain periods: 10 minutes, 1 hour, 6 hours, and 12 hours. The amount of newly synthesised collagen, both those retained in strained fascicles and released into culture media, were not significantly altered by the application of different patters of intermittent cyclic strain. The present findings may suggest that, unlike other connective tissue cells, tenocyte responses are predominantly regulated by the total number of strain cycles.

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Published date: 2009

Identifiers

Local EPrints ID: 169365
URI: http://eprints.soton.ac.uk/id/eprint/169365
ISSN: 1880-9863
PURE UUID: 6d77eac3-3a80-4244-9e99-0a401ad98664

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Date deposited: 14 Dec 2010 10:11
Last modified: 16 Jul 2019 23:49

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