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Design of a photon-counting interrogation unit for monitoring Ru2+ oxygen sensors

Design of a photon-counting interrogation unit for monitoring Ru2+ oxygen sensors
Design of a photon-counting interrogation unit for monitoring Ru2+ oxygen sensors
By combining recently available photomultiplier-based photon-counting modules with simple optics and electronics, it is possible to construct highly sensitive, yet reasonably compact, fluorescence lifetime monitors, capable of working with extremely low fluorescent-light return levels. Measurements are rapid, the unit operates with relatively little excitation cross-talk (due to temporal separation of send and return signals) and the same basic apparatus can be used as a diagnostic tool to characterise the fluorescent decay curves from layers.

The presented sensor, which is designed to interrogate Ru2+ complex indicator dyes (fluorescence lifetime in the range 3-7 microseconds), derives excitation light from an HP high-intensity blue LED source (a flash-lamp is not required). With the aid of a dichroic splitter block, a single optical fibre is used to guide excitation light to the dye and to collect the resulting fluorescence.
Austin, E.A.
5e62fbb2-313b-4368-a371-2d8cb7978109
Dakin, J.P.
490d5873-c444-423b-8d83-c49f118dc4b5
Strong, A.P.
b6d72d5e-d003-4a1e-9a5a-6a9f559ba067
Austin, E.A.
5e62fbb2-313b-4368-a371-2d8cb7978109
Dakin, J.P.
490d5873-c444-423b-8d83-c49f118dc4b5
Strong, A.P.
b6d72d5e-d003-4a1e-9a5a-6a9f559ba067

Austin, E.A., Dakin, J.P. and Strong, A.P. (2000) Design of a photon-counting interrogation unit for monitoring Ru2+ oxygen sensors. Europt(R)ode, France. 16 - 19 Apr 2000.

Record type: Conference or Workshop Item (Paper)

Abstract

By combining recently available photomultiplier-based photon-counting modules with simple optics and electronics, it is possible to construct highly sensitive, yet reasonably compact, fluorescence lifetime monitors, capable of working with extremely low fluorescent-light return levels. Measurements are rapid, the unit operates with relatively little excitation cross-talk (due to temporal separation of send and return signals) and the same basic apparatus can be used as a diagnostic tool to characterise the fluorescent decay curves from layers.

The presented sensor, which is designed to interrogate Ru2+ complex indicator dyes (fluorescence lifetime in the range 3-7 microseconds), derives excitation light from an HP high-intensity blue LED source (a flash-lamp is not required). With the aid of a dichroic splitter block, a single optical fibre is used to guide excitation light to the dye and to collect the resulting fluorescence.

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Published date: 2000
Additional Information: P82
Venue - Dates: Europt(R)ode, France, 2000-04-16 - 2000-04-19

Identifiers

Local EPrints ID: 16966
URI: http://eprints.soton.ac.uk/id/eprint/16966
PURE UUID: b4e76192-bc61-4504-bd51-8f185b0d6ea6

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Date deposited: 23 Aug 2005
Last modified: 17 Jul 2017 16:42

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