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Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources

Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources
Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources
Siderophore type chelates were detected in nutrient enriched, incubated seawater collected from different biogeographical regions of the Atlantic Ocean. Seawater was enriched with glucose and ammonium, glycine (as a source of carbon and nitrogen) or chitin and ammonium at different concentrations and incubated for up to 3 – 4 days in the dark. Siderophore type chelates were detected using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) after complexation with Ga. Samples were subsequently analysed by HPLC - electrospray ionisation mass spectrometry (HPLC
ESI-MS) in order to confirm the identity of the known siderophores, and to obtain the pseudo molecular ions of unknown siderophore type chelates. A total of 22 different siderophore type chelates were resolved in the HPLC-ICP-MS chromatograms. Ten different siderophore type chelates were identified by HPLC-ESI-MS, 3 of which had not previously been identified in nutrient enriched seawater incubations. The concentration and diversity of siderophore typechelates was highest in seawater amended with glucose. The concentrations 1 and diversity of siderophore type chelates also varied with biogeographical area in the Atlantic Ocean, with the North Atlantic Sub-tropical Gyre yielding highest concentrations in incubations, and the South Atlantic Sub-tropical Gyre and Western Tropical Atlantic yielding the highest diversity.
Iron biogeochemistry, Siderophores, Seawater, Bacteria, Atlantic Ocean
0304-4203
90-99
Mawji, Edward
36970997-a479-4cc5-8120-82a2fe000202
Gledhill, M.
da795c1e-1489-4d40-9df1-fc6bde54382d
Milton, J.A.
9e183221-d0d4-4ddb-aeba-0fdde9d31230
Zubkov, M.V.
b1dfb3a0-bcff-430c-9031-358a22b50743
Thompson, Anu
e1d5ef95-996b-45f0-9208-68a7f5fe4a21
Wolff, George A.
6b29d886-06f9-4405-8fab-33cfb436acaa
Achterberg, Eric P.
685ce961-8c45-4503-9f03-50f6561202b9
Mawji, Edward
36970997-a479-4cc5-8120-82a2fe000202
Gledhill, M.
da795c1e-1489-4d40-9df1-fc6bde54382d
Milton, J.A.
9e183221-d0d4-4ddb-aeba-0fdde9d31230
Zubkov, M.V.
b1dfb3a0-bcff-430c-9031-358a22b50743
Thompson, Anu
e1d5ef95-996b-45f0-9208-68a7f5fe4a21
Wolff, George A.
6b29d886-06f9-4405-8fab-33cfb436acaa
Achterberg, Eric P.
685ce961-8c45-4503-9f03-50f6561202b9

Mawji, Edward, Gledhill, M., Milton, J.A., Zubkov, M.V., Thompson, Anu, Wolff, George A. and Achterberg, Eric P. (2011) Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources. Marine Chemistry, 124 (1-4), 90-99. (doi:10.1016/j.marchem.2010.12.005).

Record type: Article

Abstract

Siderophore type chelates were detected in nutrient enriched, incubated seawater collected from different biogeographical regions of the Atlantic Ocean. Seawater was enriched with glucose and ammonium, glycine (as a source of carbon and nitrogen) or chitin and ammonium at different concentrations and incubated for up to 3 – 4 days in the dark. Siderophore type chelates were detected using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) after complexation with Ga. Samples were subsequently analysed by HPLC - electrospray ionisation mass spectrometry (HPLC
ESI-MS) in order to confirm the identity of the known siderophores, and to obtain the pseudo molecular ions of unknown siderophore type chelates. A total of 22 different siderophore type chelates were resolved in the HPLC-ICP-MS chromatograms. Ten different siderophore type chelates were identified by HPLC-ESI-MS, 3 of which had not previously been identified in nutrient enriched seawater incubations. The concentration and diversity of siderophore typechelates was highest in seawater amended with glucose. The concentrations 1 and diversity of siderophore type chelates also varied with biogeographical area in the Atlantic Ocean, with the North Atlantic Sub-tropical Gyre yielding highest concentrations in incubations, and the South Atlantic Sub-tropical Gyre and Western Tropical Atlantic yielding the highest diversity.

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Published date: 20 March 2011
Keywords: Iron biogeochemistry, Siderophores, Seawater, Bacteria, Atlantic Ocean
Organisations: Ocean and Earth Science, Marine Biogeochemistry, National Oceanography Centre,Southampton

Identifiers

Local EPrints ID: 170213
URI: https://eprints.soton.ac.uk/id/eprint/170213
ISSN: 0304-4203
PURE UUID: 8d435baf-c1c2-48bd-afdc-f49ebec12d9f

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Date deposited: 04 Jan 2011 14:57
Last modified: 02 Dec 2019 21:18

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