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Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources

Mawji, Edward, Gledhill, M., Milton, J.A., Zubkov, M.V., Thompson, Anu, Wolff, George A. and Achterberg, Eric P. (2011) Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources Marine Chemistry, 124, (1-4), pp. 90-99. (doi:10.1016/j.marchem.2010.12.005).

Record type: Article


Siderophore type chelates were detected in nutrient enriched, incubated seawater collected from different biogeographical regions of the Atlantic Ocean. Seawater was enriched with glucose and ammonium, glycine (as a source of carbon and nitrogen) or chitin and ammonium at different concentrations and incubated for up to 3 – 4 days in the dark. Siderophore type chelates were detected using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) after complexation with Ga. Samples were subsequently analysed by HPLC - electrospray ionisation mass spectrometry (HPLC
ESI-MS) in order to confirm the identity of the known siderophores, and to obtain the pseudo molecular ions of unknown siderophore type chelates. A total of 22 different siderophore type chelates were resolved in the HPLC-ICP-MS chromatograms. Ten different siderophore type chelates were identified by HPLC-ESI-MS, 3 of which had not previously been identified in nutrient enriched seawater incubations. The concentration and diversity of siderophore typechelates was highest in seawater amended with glucose. The concentrations 1 and diversity of siderophore type chelates also varied with biogeographical area in the Atlantic Ocean, with the North Atlantic Sub-tropical Gyre yielding highest concentrations in incubations, and the South Atlantic Sub-tropical Gyre and Western Tropical Atlantic yielding the highest diversity.

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Published date: 20 March 2011
Keywords: Iron biogeochemistry, Siderophores, Seawater, Bacteria, Atlantic Ocean
Organisations: Ocean and Earth Science, Marine Biogeochemistry, National Oceanography Centre,Southampton


Local EPrints ID: 170213
ISSN: 0304-4203
PURE UUID: 8d435baf-c1c2-48bd-afdc-f49ebec12d9f

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Date deposited: 04 Jan 2011 14:57
Last modified: 18 Jul 2017 12:17

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Author: Edward Mawji
Author: M. Gledhill
Author: J.A. Milton
Author: M.V. Zubkov
Author: Anu Thompson
Author: George A. Wolff

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