The University of Southampton
University of Southampton Institutional Repository

Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification

Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification
Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification
We describe here a simple device for dielectrophoretic concentration of marine microalga Karenia brevis non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including in situ harmful algal bloom detection, transcriptomics and point-of-care diagnostics.
electroporation, dielectrophoresis, karenia brevis, rna extraction, rna amplification, dinoflagellate cysts
1742-5689
601-608
Bahi, M.M.
97860b79-de5c-443c-b862-45b7bb46bda7
Tsaloglou, M-N.
99ab30ba-15da-4d25-86ba-608d127f8369
Mowlem, M.
6f633ca2-298f-48ee-a025-ce52dd62124f
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Bahi, M.M.
97860b79-de5c-443c-b862-45b7bb46bda7
Tsaloglou, M-N.
99ab30ba-15da-4d25-86ba-608d127f8369
Mowlem, M.
6f633ca2-298f-48ee-a025-ce52dd62124f
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Bahi, M.M., Tsaloglou, M-N., Mowlem, M. and Morgan, H. (2011) Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification. Journal of the Royal Society Interface, 8 (57), 601-608. (doi:10.1098/rsif.2010.0445). (PMID:21084344)

Record type: Article

Abstract

We describe here a simple device for dielectrophoretic concentration of marine microalga Karenia brevis non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including in situ harmful algal bloom detection, transcriptomics and point-of-care diagnostics.

Full text not available from this repository.

More information

e-pub ahead of print date: 17 November 2010
Published date: April 2011
Keywords: electroporation, dielectrophoresis, karenia brevis, rna extraction, rna amplification, dinoflagellate cysts
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 171055
URI: https://eprints.soton.ac.uk/id/eprint/171055
ISSN: 1742-5689
PURE UUID: 045216d4-2e54-401c-a1f3-6897987cb10f
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 13 Jan 2011 09:21
Last modified: 20 Jul 2019 00:59

Export record

Altmetrics

Contributors

Author: M.M. Bahi
Author: M-N. Tsaloglou
Author: M. Mowlem
Author: H. Morgan ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×