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Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification

Bahi, M.M., Tsaloglou, M-N., Mowlem, M. and Morgan, H. (2011) Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification Journal of the Royal Society Interface, 8, (57), pp. 601-608. (PMID:21084344).

Record type: Article

Abstract

We describe here a simple device for dielectrophoretic concentration of marine microalga Karenia brevis non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including in situ harmful algal bloom detection, transcriptomics and point-of-care diagnostics.

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More information

e-pub ahead of print date: 17 November 2010
Published date: April 2011
Keywords: electroporation, dielectrophoresis, karenia brevis, rna extraction, rna amplification, dinoflagellate cysts
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 171055
URI: http://eprints.soton.ac.uk/id/eprint/171055
ISSN: 1742-5689
PURE UUID: 045216d4-2e54-401c-a1f3-6897987cb10f
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 13 Jan 2011 09:21
Last modified: 13 Oct 2017 13:41

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