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Anti-biofouling coatings for optical fiber sensors

Anti-biofouling coatings for optical fiber sensors
Anti-biofouling coatings for optical fiber sensors
One of the most relevant problems of using optical fiber sensors in real-world environments is surface fouling, that is the cumulative build-up of undesirable material on the working surface of the sensor.

One of the scopes of the Brite-EuRam Project 'BOSS' was the development of non-toxic, optically-transparent and biologically-inert materials, suitable to inhibiting or reducing bio-fouling, to be used as coatings for optical and optical fiber sensors.

The present paper presents the results of tests of anti-biofouling coated fiber optic probes for reflectance spectroscopy in blood-simulating 'foul' media, that are Bovine Serum Albumin (BSA), Fibrinogen and Platelet Enriched plasma (PEP). The anti-biofouling coating, a proprietary invention of Biocompatibles Ltd., was a cross-linkable polymer with silane functionality to improve adhesion to silica-containing substrates.

Two identical fiber optic probes for reflectance spectroscopy were considered, one of which was coated by means of PC-1036 layer. Both probes were optically excited and the signals were continuously detected while immersing the probes in a by-pass flow cell used as dynamic test environment. After 24-hour testing, a visual check of both probes was carried out by means of optical microscopy.

All tests in BSA, Fibrinogen and PEP showed that PC-1036 coating was suitable to avoid build-up of biological material. In fact optical signal variations of non-coated probes showed fluctuations in the 6-20% range, while coated probes exhibited a nearly-stable optical signal. These results were confirmed also by microscopic check, which showed adhesions of biological material on non-coated probes.

The figure below shows the end surfaces of two optical fiber couples after 24-hour testing in BSA. Fibers at the left were anti-biofouling coated and appeared perfectly clean: only the shadow of the coating was visible. Fibers at the right were un-coated and showed a relevant adhesion of biological material. Similar images were obtained by testings in Fibrinogen and PEP.

Photos: 1506_BSA

Acknowledgements
This work was funded by Brite-EuRam EU Project 'BOSS', contract # BRPR-CT97-0485.
Mignani, A.G.
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Bizzarri, P.
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Driver, M.
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Palmer, R.
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Liefeith, K.
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Hildebrand, G.
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Dakin, J.P.
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Mignani, A.G.
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Bizzarri, P.
350dc1af-2586-4c74-aabf-f7f2e60372f2
Driver, M.
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Palmer, R.
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Liefeith, K.
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Hildebrand, G.
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Dakin, J.P.
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Mignani, A.G., Bizzarri, P., Driver, M., Palmer, R., Liefeith, K., Hildebrand, G. and Dakin, J.P. (2001) Anti-biofouling coatings for optical fiber sensors. Fiber Optic Sensor Technology and Applications, Newton, United States. 30 Oct - 01 Nov 2001.

Record type: Conference or Workshop Item (Paper)

Abstract

One of the most relevant problems of using optical fiber sensors in real-world environments is surface fouling, that is the cumulative build-up of undesirable material on the working surface of the sensor.

One of the scopes of the Brite-EuRam Project 'BOSS' was the development of non-toxic, optically-transparent and biologically-inert materials, suitable to inhibiting or reducing bio-fouling, to be used as coatings for optical and optical fiber sensors.

The present paper presents the results of tests of anti-biofouling coated fiber optic probes for reflectance spectroscopy in blood-simulating 'foul' media, that are Bovine Serum Albumin (BSA), Fibrinogen and Platelet Enriched plasma (PEP). The anti-biofouling coating, a proprietary invention of Biocompatibles Ltd., was a cross-linkable polymer with silane functionality to improve adhesion to silica-containing substrates.

Two identical fiber optic probes for reflectance spectroscopy were considered, one of which was coated by means of PC-1036 layer. Both probes were optically excited and the signals were continuously detected while immersing the probes in a by-pass flow cell used as dynamic test environment. After 24-hour testing, a visual check of both probes was carried out by means of optical microscopy.

All tests in BSA, Fibrinogen and PEP showed that PC-1036 coating was suitable to avoid build-up of biological material. In fact optical signal variations of non-coated probes showed fluctuations in the 6-20% range, while coated probes exhibited a nearly-stable optical signal. These results were confirmed also by microscopic check, which showed adhesions of biological material on non-coated probes.

The figure below shows the end surfaces of two optical fiber couples after 24-hour testing in BSA. Fibers at the left were anti-biofouling coated and appeared perfectly clean: only the shadow of the coating was visible. Fibers at the right were un-coated and showed a relevant adhesion of biological material. Similar images were obtained by testings in Fibrinogen and PEP.

Photos: 1506_BSA

Acknowledgements
This work was funded by Brite-EuRam EU Project 'BOSS', contract # BRPR-CT97-0485.

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More information

Published date: 2001
Additional Information: SPIE
Venue - Dates: Fiber Optic Sensor Technology and Applications, Newton, United States, 2001-10-30 - 2001-11-01

Identifiers

Local EPrints ID: 17124
URI: http://eprints.soton.ac.uk/id/eprint/17124
PURE UUID: f61bdecd-3691-44f9-b94e-632da8cc80b2

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Date deposited: 19 Sep 2005
Last modified: 07 Jan 2022 22:00

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Contributors

Author: A.G. Mignani
Author: P. Bizzarri
Author: M. Driver
Author: R. Palmer
Author: K. Liefeith
Author: G. Hildebrand
Author: J.P. Dakin

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