Generic protease detection technology for monitoring periodontal disease


Zheng, Xinwei, Cook, Joseph P., Watkinson, Michael, Yang, Shoufeng, Douglas, Ian, Rawlinson, Andrew and Krause, Steffi (2011) Generic protease detection technology for monitoring periodontal disease Faraday Discussions, 149, pp. 37-47. (doi:10.1039/C005364C).

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Description/Abstract

Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.


Item Type: Article
Digital Object Identifier (DOI): doi:10.1039/C005364C
ISSNs: 0301-7249 (print)
Subjects:

Organisations: Engineering Mats & Surface Engineerg Gp
ePrint ID: 172045
Date :
Date Event
2011Published
Date Deposited: 24 Jan 2011 11:20
Last Modified: 18 Apr 2017 03:24
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/172045

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