The University of Southampton
University of Southampton Institutional Repository

Invariant Valpha14 chain NKT cells promote Plasmodium berghei circumsporozoite protein-specific gamma interferon- and tumor necrosis factor alpha-producing CD8+ T cells in the liver after poxvirus vaccination of mice

Record type: Article


Understanding the protective mechanism in the liver induced by recombinant vaccines against the pre-erythrocytic stages of malaria is important for vaccine development. Most studies in mice have focused on splenic and peripheral blood T cells and identified gamma interferon (IFN-)-producing CD8+ T cells as correlates of protection, which can be induced by prime-boost vaccination with recombinant poxviruses. Invariant natural killer T (V14iNKT) cells can also protect against liver stage malaria, when activated, and are abundant in the liver. Since poxviruses have nonspecific immunomodulating effects, which are incompletely understood, we investigated whether recombinant poxviruses affect the protective properties of hepatic V14iNKT cells and thus vaccine efficacy. We show that intradermal vaccination with recombinant poxviruses activated V14iNKT cells and NK cells in the livers of BALB/c mice while inducing IFN-- and tumor necrosis factor alpha (TNF-)-producing pre-erythrocytic stage antigen-specific CD8+ T cells. Greater numbers of hepatic V14iNKT cells secreted interleukin-4 than IFN-. Vaccinated V14iNKT-cell-deficient mice had lower, but still protective levels of hepatic and splenic IFN-+ and TNF-+ CD8+ T cells and better protection rates later after challenge with Plasmodium berghei sporozoites. Therefore, vaccine-activated hepatic V14iNKT cells help in generating specific T cells but are not required for protection induced by recombinant poxviruses. Furthermore, double-positive INF-+/TNF-+ CD8+ T cells were enriched in protected livers, suggesting that cells expressing both of these cytokines may be most relevant for protection.

Full text not available from this repository.

Citation

Korten, Simone, Anderson, Richard J., Hannan, Carolyn M., Sheu, Eric G., Sinden, Robert, Gadola, Stephan, Taniguchi, Masaru and Hill, Adrian V, (2005) Invariant Valpha14 chain NKT cells promote Plasmodium berghei circumsporozoite protein-specific gamma interferon- and tumor necrosis factor alpha-producing CD8+ T cells in the liver after poxvirus vaccination of mice Infection and Immunity, 73, (2), pp. 849-858. (doi:10.1128/IAI.73.2.849-858.2005). (PMID:15664925).

More information

Published date: February 2005

Identifiers

Local EPrints ID: 175447
URI: http://eprints.soton.ac.uk/id/eprint/175447
ISSN: 0019-9567
PURE UUID: 8566e18b-9f34-49f8-8b31-922e3986c383

Catalogue record

Date deposited: 23 Feb 2011 14:33
Last modified: 18 Jul 2017 12:09

Export record

Altmetrics

Contributors

Author: Simone Korten
Author: Richard J. Anderson
Author: Carolyn M. Hannan
Author: Eric G. Sheu
Author: Robert Sinden
Author: Stephan Gadola
Author: Masaru Taniguchi
Author: Adrian V, Hill

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×