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The in vitro differentiation of rat neural stem cells into an insulin-expressing phenotype

The in vitro differentiation of rat neural stem cells into an insulin-expressing phenotype
The in vitro differentiation of rat neural stem cells into an insulin-expressing phenotype
Mature beta-cells and nerve cells share many functional similarities despite originating from different embryonic germ layers. The aim of this study was to investigate the potential of neural stem cells (NSCs), isolated from foetal rat brain, as a starting material from which to generate functionally responsive, insulin-containing cells. Our results demonstrated that NSCs can be significantly expanded in vitro and can be induced to express increased preproinsulin mRNA levels. In addition, these NSC-derived cells expressed transcriptional and functional elements associated with a mature beta-cell phenotype. The differentiated cells showed functional responses typical of pancreatic beta-cells, including glucose-dependent increases in metabolism and rapid elevations in intracellular Ca(2+) in response to the sulphonylurea tolbutamide or to increased glucose concentration. These results suggest that NSCs may have potential as a starting material from which to generate beta-cell surrogates for the treatment of patients with Type 1 diabetes mellitus.
neural stem cell, pancreatic ?-cell, insulin gene, signal recognition, islet transplantation, diabetes mellitus
0006-291X
570-577
Burns, Chris J.
be3ec851-d9f5-4e79-b261-ae302ca9b382
Minger, Stephen L.
daa3154e-3a75-430a-a44b-6c9ec75fe4dc
Hall, Sara
44e40997-ff4b-45a2-8e24-992317b770ac
Milne, Helen
5ecd9a32-52d0-4b77-9f1d-536213baaf03
Ramracheya, Reshma D.
9f4ba776-f2a3-48c5-87dc-b697b2752e53
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Persaud, Shanta J.
e4ed447e-778b-4539-8af9-39a03afc8c6c
Jones, Peter M.
38a5ea85-3372-4389-bbf0-f4330dd86f6d
Burns, Chris J.
be3ec851-d9f5-4e79-b261-ae302ca9b382
Minger, Stephen L.
daa3154e-3a75-430a-a44b-6c9ec75fe4dc
Hall, Sara
44e40997-ff4b-45a2-8e24-992317b770ac
Milne, Helen
5ecd9a32-52d0-4b77-9f1d-536213baaf03
Ramracheya, Reshma D.
9f4ba776-f2a3-48c5-87dc-b697b2752e53
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Persaud, Shanta J.
e4ed447e-778b-4539-8af9-39a03afc8c6c
Jones, Peter M.
38a5ea85-3372-4389-bbf0-f4330dd86f6d

Burns, Chris J., Minger, Stephen L., Hall, Sara, Milne, Helen, Ramracheya, Reshma D., Evans, Nicholas D., Persaud, Shanta J. and Jones, Peter M. (2005) The in vitro differentiation of rat neural stem cells into an insulin-expressing phenotype. Biochemical and Biophysical Research Communications, 326 (3), 570-577. (doi:10.1016/j.bbrc.2004.11.062). (PMID:15596137)

Record type: Article

Abstract

Mature beta-cells and nerve cells share many functional similarities despite originating from different embryonic germ layers. The aim of this study was to investigate the potential of neural stem cells (NSCs), isolated from foetal rat brain, as a starting material from which to generate functionally responsive, insulin-containing cells. Our results demonstrated that NSCs can be significantly expanded in vitro and can be induced to express increased preproinsulin mRNA levels. In addition, these NSC-derived cells expressed transcriptional and functional elements associated with a mature beta-cell phenotype. The differentiated cells showed functional responses typical of pancreatic beta-cells, including glucose-dependent increases in metabolism and rapid elevations in intracellular Ca(2+) in response to the sulphonylurea tolbutamide or to increased glucose concentration. These results suggest that NSCs may have potential as a starting material from which to generate beta-cell surrogates for the treatment of patients with Type 1 diabetes mellitus.

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More information

Published date: 21 January 2005
Keywords: neural stem cell, pancreatic ?-cell, insulin gene, signal recognition, islet transplantation, diabetes mellitus

Identifiers

Local EPrints ID: 176163
URI: https://eprints.soton.ac.uk/id/eprint/176163
ISSN: 0006-291X
PURE UUID: 0830a13e-cd3f-4200-b4f3-3e67540a8973

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Date deposited: 08 Mar 2011 13:39
Last modified: 18 Jul 2017 12:08

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Contributors

Author: Chris J. Burns
Author: Stephen L. Minger
Author: Sara Hall
Author: Helen Milne
Author: Reshma D. Ramracheya
Author: Shanta J. Persaud
Author: Peter M. Jones

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