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Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulate transcription of bone morphogenic proteins 2 and 4 in rat osteoblasts in vitro

Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulate transcription of bone morphogenic proteins 2 and 4 in rat osteoblasts in vitro
Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulate transcription of bone morphogenic proteins 2 and 4 in rat osteoblasts in vitro
Pulsed electromagnetic fields (PEMF) are successfully employed in the treatment of a variety of orthopaedic conditions, particularly delayed and nonunion fractures. In this study, we examined PEMF effects onin vitroosteogenesis by bone nodule formation and on mRNA expression of bone morphogenetic proteins 2 and 4 by reverse-transcriptase polymerase chain reaction (RT-PCR) in cultured rat calvarial osteoblasts. PEMF exposure induced a significant increase in both the number (39% over unexposed controls) and size (70% larger compared to unexposed controls) of bone-like nodules formed. PEMF also induced an increase in the levels of BMP-2 and BMP-4 mRNA in comparison to controls. This effect was directly related to the duration of PEMF exposure. This study shows that clinically applied PEMF have a reproducible osteogenic effectin vitroand simultaneously induce BMP-2 and -4 mRNA transcription. This supports the concept that the two effects are related.

0006-291X
458-461
Bodamyali, T.
1ac1f466-58d4-4ac8-8cac-264b6dd1f384
Bhatt, B.
257901d9-5852-434b-ae5f-0846b9366370
Hughes, F.J.
3b9f88ce-c22f-4893-b951-e662aa5d5525
Winrow, V.R.
d65322b5-851d-46e4-a7e8-3cd914b3d93a
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Simon, B.
1a713722-984e-45dc-8b04-9044b28f127b
Abbott, J.
fe4c1f12-b208-4d4a-bdfb-398eb93fc35d
Blake, D.R.
6e4cc35d-a9b8-459e-bd39-f36501dd735c
Stevens, C.R.
70732c45-74f8-4f00-9f5b-f729b7896060
Bodamyali, T.
1ac1f466-58d4-4ac8-8cac-264b6dd1f384
Bhatt, B.
257901d9-5852-434b-ae5f-0846b9366370
Hughes, F.J.
3b9f88ce-c22f-4893-b951-e662aa5d5525
Winrow, V.R.
d65322b5-851d-46e4-a7e8-3cd914b3d93a
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Simon, B.
1a713722-984e-45dc-8b04-9044b28f127b
Abbott, J.
fe4c1f12-b208-4d4a-bdfb-398eb93fc35d
Blake, D.R.
6e4cc35d-a9b8-459e-bd39-f36501dd735c
Stevens, C.R.
70732c45-74f8-4f00-9f5b-f729b7896060

Bodamyali, T., Bhatt, B., Hughes, F.J., Winrow, V.R., Kanczler, J.M., Simon, B., Abbott, J., Blake, D.R. and Stevens, C.R. (1998) Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulate transcription of bone morphogenic proteins 2 and 4 in rat osteoblasts in vitro. Biochemical and Biophysical Research Communications, 250 (2), 458-461. (doi:10.1006/bbrc.1998.9243).

Record type: Article

Abstract

Pulsed electromagnetic fields (PEMF) are successfully employed in the treatment of a variety of orthopaedic conditions, particularly delayed and nonunion fractures. In this study, we examined PEMF effects onin vitroosteogenesis by bone nodule formation and on mRNA expression of bone morphogenetic proteins 2 and 4 by reverse-transcriptase polymerase chain reaction (RT-PCR) in cultured rat calvarial osteoblasts. PEMF exposure induced a significant increase in both the number (39% over unexposed controls) and size (70% larger compared to unexposed controls) of bone-like nodules formed. PEMF also induced an increase in the levels of BMP-2 and BMP-4 mRNA in comparison to controls. This effect was directly related to the duration of PEMF exposure. This study shows that clinically applied PEMF have a reproducible osteogenic effectin vitroand simultaneously induce BMP-2 and -4 mRNA transcription. This supports the concept that the two effects are related.

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Published date: September 1998

Identifiers

Local EPrints ID: 176997
URI: http://eprints.soton.ac.uk/id/eprint/176997
ISSN: 0006-291X
PURE UUID: 4c1b5af2-0caf-4b2d-8170-429329303e35
ORCID for J.M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414

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Date deposited: 14 Mar 2011 09:40
Last modified: 11 Jul 2024 01:43

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Contributors

Author: T. Bodamyali
Author: B. Bhatt
Author: F.J. Hughes
Author: V.R. Winrow
Author: J.M. Kanczler ORCID iD
Author: B. Simon
Author: J. Abbott
Author: D.R. Blake
Author: C.R. Stevens

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