Osteogenesis on surface selective laser sintered bioresorbable scaffolds

Bagratashvili, Victor, Antonov, E.N., Howdle, S.M., Kanczler, J.M., Mirmalek-Sani, S., Popov, V.K., Oreffo, R.O. and Upton, C. (2008) Osteogenesis on surface selective laser sintered bioresorbable scaffolds. Katashev, Alexei, Dekhtyar, Yuri and Spigulis, Janis (eds.) In 14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. vol. 20, Springer. pp. 12-15 . (doi:10.1007/978-3-540-69367-3_4).

Abstract

In this study we have used a novel surface selective laser sintering (SSLS) technique to develop CAD/CAM designed scaffolds for bone tissue engineering. SSLS polylactic acid scaffolds were evaluated in vitro and in vivo as templates for human fetal femur-derived cell and adult human bone marrow stromal cell osteogenesis. Both cell types were cultured successfully on SSLS scaffolds with an increase in expression of alkaline phosphatase activity. Cell in-growth and Alcian blue/Sirius red positive staining of matrix deposition were observed on SSLS scaffolds in vitro in basal medium and osteogenic culture conditions. Similar results were observed in vivo with type I collagen expressed by cells on the scaffolds. In the critical sized femur segmental defect, SSLS scaffolds seeded with the cells enhanced significantly bone tissue regeneration

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Contributors

Author: J.M. Kanczler

Author: R.O. Oreffo

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