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Osteogenesis on surface selective laser sintered bioresorbable scaffolds

Osteogenesis on surface selective laser sintered bioresorbable scaffolds
Osteogenesis on surface selective laser sintered bioresorbable scaffolds
In this study we have used a novel surface selective laser sintering (SSLS) technique to develop CAD/CAM designed scaffolds for bone tissue engineering. SSLS polylactic acid scaffolds were evaluated in vitro and in vivo as templates for human fetal femur-derived cell and adult human bone marrow stromal cell osteogenesis. Both cell types were cultured successfully on SSLS scaffolds with an increase in expression of alkaline phosphatase activity. Cell in-growth and Alcian blue/Sirius red positive staining of matrix deposition were observed on SSLS scaffolds in vitro in basal medium and osteogenic culture conditions. Similar results were observed in vivo with type I collagen expressed by cells on the scaffolds. In the critical sized femur segmental defect, SSLS scaffolds seeded with the cells enhanced significantly bone tissue regeneration
978-3-540-69366-6
12-15
Springer
Bagratashvili, Victor
8985d6c4-5844-4c9c-9d0f-858ad169d808
Antonov, E.N.
e4fe1580-e0e6-4164-8e93-ea1e800dda56
Howdle, S.M.
9aaf52a9-58ae-4811-947a-0498f153cfa5
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Mirmalek-Sani, S.
203c64a3-8d4a-4acc-9fe0-14fbd90fa2c4
Popov, V.K.
a6c90514-f4a2-4ec2-a785-6cd304949ec4
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Upton, C.
762ced8b-90c9-48b2-93df-2fe8b1c45d45
Katashev, Alexei
55d9718c-1dc1-4908-b86e-64061953309e
Dekhtyar, Yuri
9a33b7c7-563c-4e0f-91c6-bec4de97382a
Spigulis, Janis
ad388f81-f2de-4225-99ee-d38ece7ae32f
Bagratashvili, Victor
8985d6c4-5844-4c9c-9d0f-858ad169d808
Antonov, E.N.
e4fe1580-e0e6-4164-8e93-ea1e800dda56
Howdle, S.M.
9aaf52a9-58ae-4811-947a-0498f153cfa5
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Mirmalek-Sani, S.
203c64a3-8d4a-4acc-9fe0-14fbd90fa2c4
Popov, V.K.
a6c90514-f4a2-4ec2-a785-6cd304949ec4
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Upton, C.
762ced8b-90c9-48b2-93df-2fe8b1c45d45
Katashev, Alexei
55d9718c-1dc1-4908-b86e-64061953309e
Dekhtyar, Yuri
9a33b7c7-563c-4e0f-91c6-bec4de97382a
Spigulis, Janis
ad388f81-f2de-4225-99ee-d38ece7ae32f

Bagratashvili, Victor, Antonov, E.N., Howdle, S.M., Kanczler, J.M., Mirmalek-Sani, S., Popov, V.K., Oreffo, R.O. and Upton, C. (2008) Osteogenesis on surface selective laser sintered bioresorbable scaffolds. Katashev, Alexei, Dekhtyar, Yuri and Spigulis, Janis (eds.) In 14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. vol. 20, Springer. pp. 12-15 . (doi:10.1007/978-3-540-69367-3_4).

Record type: Conference or Workshop Item (Paper)

Abstract

In this study we have used a novel surface selective laser sintering (SSLS) technique to develop CAD/CAM designed scaffolds for bone tissue engineering. SSLS polylactic acid scaffolds were evaluated in vitro and in vivo as templates for human fetal femur-derived cell and adult human bone marrow stromal cell osteogenesis. Both cell types were cultured successfully on SSLS scaffolds with an increase in expression of alkaline phosphatase activity. Cell in-growth and Alcian blue/Sirius red positive staining of matrix deposition were observed on SSLS scaffolds in vitro in basal medium and osteogenic culture conditions. Similar results were observed in vivo with type I collagen expressed by cells on the scaffolds. In the critical sized femur segmental defect, SSLS scaffolds seeded with the cells enhanced significantly bone tissue regeneration

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More information

Published date: June 2008
Venue - Dates: 14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, Latvia, 2008-06-16 - 2008-06-18

Identifiers

Local EPrints ID: 176999
URI: https://eprints.soton.ac.uk/id/eprint/176999
ISBN: 978-3-540-69366-6
PURE UUID: 8e7206d6-8a3d-4ebb-9dd4-00896593a818
ORCID for J.M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 14 Mar 2011 08:47
Last modified: 14 Mar 2019 01:48

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Contributors

Author: Victor Bagratashvili
Author: E.N. Antonov
Author: S.M. Howdle
Author: J.M. Kanczler ORCID iD
Author: S. Mirmalek-Sani
Author: V.K. Popov
Author: R.O. Oreffo ORCID iD
Author: C. Upton
Editor: Alexei Katashev
Editor: Yuri Dekhtyar
Editor: Janis Spigulis

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