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The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer

The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer
Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly method allows fine control over the film thickness, enzyme loading, osmium and glucose concentrations with good reproducibility from electrode to electrode. In the analysis we combine the use of approximate analytical expressions with digital simulation to fit the data from an extensive set of experiments. The analysis shows a thickness dependence of the fraction of “wired enzyme molecules” and second order enzyme re-oxidation rate constant for thin films (below 300 nm) following changes in the multilayer film structure. For films thicker than 300 nm the kinetic data approach that of a redox hydrogel.

enzyme electrode, kinetics, glucose oxidase, osmium redox polymer, layer-by-layer assembly
1572-6657
24-32
Flexer, V.
da447235-0802-405a-876a-02d918abf2ab
Calvo, E.J.
66bc120f-b6c6-4f83-af3b-74dbafce0118
Bartlett, P.N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Flexer, V.
da447235-0802-405a-876a-02d918abf2ab
Calvo, E.J.
66bc120f-b6c6-4f83-af3b-74dbafce0118
Bartlett, P.N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Flexer, V., Calvo, E.J. and Bartlett, P.N. (2010) The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer. [in special issue: Special Issue Dedicated to Laurence M. Peter] Journal of Electroanalytical Chemistry, 646 (1-2), 24-32. (doi:10.1016/j.jelechem.2009.11.017).

Record type: Article

Abstract

Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly method allows fine control over the film thickness, enzyme loading, osmium and glucose concentrations with good reproducibility from electrode to electrode. In the analysis we combine the use of approximate analytical expressions with digital simulation to fit the data from an extensive set of experiments. The analysis shows a thickness dependence of the fraction of “wired enzyme molecules” and second order enzyme re-oxidation rate constant for thin films (below 300 nm) following changes in the multilayer film structure. For films thicker than 300 nm the kinetic data approach that of a redox hydrogel.

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More information

Published date: 15 July 2010
Keywords: enzyme electrode, kinetics, glucose oxidase, osmium redox polymer, layer-by-layer assembly

Identifiers

Local EPrints ID: 177977
URI: http://eprints.soton.ac.uk/id/eprint/177977
ISSN: 1572-6657
PURE UUID: d381ebcc-1647-4603-8716-4375b1bbc810
ORCID for P.N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 23 Mar 2011 11:50
Last modified: 03 Dec 2019 02:04

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Contributors

Author: V. Flexer
Author: E.J. Calvo
Author: P.N. Bartlett ORCID iD

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