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Monolayer anthracene and anthraquinone modified electrodes as platforms for Trametes hirsuta laccase immobilisation

Monolayer anthracene and anthraquinone modified electrodes as platforms for Trametes hirsuta laccase immobilisation
Monolayer anthracene and anthraquinone modified electrodes as platforms for Trametes hirsuta laccase immobilisation
Surface modification techniques are essential to the construction of enzyme based elements of biofuel cells and biosensors. In this article we report on the preparation and characterisation of modified carbon electrodes which were used as supports for the immobilisation of laccase from Trametes hirsuta. The electrodes were electrochemically modified with diamine or diazonium linkers followed by attachment of either anthracene or anthraquinone head groups using solid phase chemical methodology. These well defined surfaces were found to effectively bind laccase and to provide direct electrical contact to the enzyme active site, as evidenced by XPS, EIS and voltammetry, respectively. The influence of the type of linker and head group on enzyme binding and bioelectrocatalytic activity are evaluated.


1463-9076
10018-10026
Sosna, Maciej
b089fe7e-6f5b-4e30-a43c-0cde0aa1fac4
Chrétien, Jean-Mathieu
ae3cb8ea-7cf0-4ce1-a1d0-1a6fdfe7e311
Kilburn, Jeremy D.
e64ded70-825a-40ec-816b-c4605e007e7a
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Sosna, Maciej
b089fe7e-6f5b-4e30-a43c-0cde0aa1fac4
Chrétien, Jean-Mathieu
ae3cb8ea-7cf0-4ce1-a1d0-1a6fdfe7e311
Kilburn, Jeremy D.
e64ded70-825a-40ec-816b-c4605e007e7a
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Sosna, Maciej, Chrétien, Jean-Mathieu, Kilburn, Jeremy D. and Bartlett, Philip N. (2010) Monolayer anthracene and anthraquinone modified electrodes as platforms for Trametes hirsuta laccase immobilisation. Physical Chemistry Chemical Physics, 12 (34), 10018-10026. (doi:10.1039/c0cp00305k).

Record type: Article

Abstract

Surface modification techniques are essential to the construction of enzyme based elements of biofuel cells and biosensors. In this article we report on the preparation and characterisation of modified carbon electrodes which were used as supports for the immobilisation of laccase from Trametes hirsuta. The electrodes were electrochemically modified with diamine or diazonium linkers followed by attachment of either anthracene or anthraquinone head groups using solid phase chemical methodology. These well defined surfaces were found to effectively bind laccase and to provide direct electrical contact to the enzyme active site, as evidenced by XPS, EIS and voltammetry, respectively. The influence of the type of linker and head group on enzyme binding and bioelectrocatalytic activity are evaluated.


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Published date: 2010

Identifiers

Local EPrints ID: 177981
URI: http://eprints.soton.ac.uk/id/eprint/177981
ISSN: 1463-9076
PURE UUID: f906fab1-078d-4d93-bcf2-3c4304f97c4a
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

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Date deposited: 22 Mar 2011 11:44
Last modified: 26 Nov 2019 02:04

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