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Partitioning of ssRNA molecules between preformed monolithic H(II) liquid crystalline phases of lipids and supernatant isotropic phases

Partitioning of ssRNA molecules between preformed monolithic H(II) liquid crystalline phases of lipids and supernatant isotropic phases
Partitioning of ssRNA molecules between preformed monolithic H(II) liquid crystalline phases of lipids and supernatant isotropic phases
The interaction of nucleic acids with the nanoarchitectures formed by lipidic systems is a new area of research that may offer insights into the functioning of genetic materials in vivo. Here we report that ssRNA has a strong preference to reside in isotropic solution rather than in inverse hexagonal (HII) liquid crystalline phases. This is in contrast to dsDNA, which becomes localized in the pores of the HII phase. The RNA that does associate with the external surfaces of the HII phase appears to form an accretion layer, tens of molecules thick, but this layer still allows the transcription of dsDNA that resides within the pores of the phase.
1525-7797
3022-3027
Wilson, Richard J.
7f511c5c-11b7-455a-a1fb-1bc476be1a64
Tyas, Sarah R.
b58657f7-3aa9-4a83-b8a8-5cfa85db592d
Black, Camilla F.
17a9e6f0-03fe-4769-8455-c876f84316a1
Dymond, Marcus K.
e180765b-039e-47ff-9841-ce6a5123a519
Attard, George S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36
Wilson, Richard J.
7f511c5c-11b7-455a-a1fb-1bc476be1a64
Tyas, Sarah R.
b58657f7-3aa9-4a83-b8a8-5cfa85db592d
Black, Camilla F.
17a9e6f0-03fe-4769-8455-c876f84316a1
Dymond, Marcus K.
e180765b-039e-47ff-9841-ce6a5123a519
Attard, George S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36

Wilson, Richard J., Tyas, Sarah R., Black, Camilla F., Dymond, Marcus K. and Attard, George S. (2010) Partitioning of ssRNA molecules between preformed monolithic H(II) liquid crystalline phases of lipids and supernatant isotropic phases. Biomacromolecules, 11 (11), 3022-3027. (doi:10.1021/bm1008469).

Record type: Article

Abstract

The interaction of nucleic acids with the nanoarchitectures formed by lipidic systems is a new area of research that may offer insights into the functioning of genetic materials in vivo. Here we report that ssRNA has a strong preference to reside in isotropic solution rather than in inverse hexagonal (HII) liquid crystalline phases. This is in contrast to dsDNA, which becomes localized in the pores of the HII phase. The RNA that does associate with the external surfaces of the HII phase appears to form an accretion layer, tens of molecules thick, but this layer still allows the transcription of dsDNA that resides within the pores of the phase.

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More information

e-pub ahead of print date: 7 October 2010
Published date: 7 October 2010

Identifiers

Local EPrints ID: 178345
URI: http://eprints.soton.ac.uk/id/eprint/178345
ISSN: 1525-7797
PURE UUID: 674719ad-7d87-4d5e-a480-8a0d14f70e5f
ORCID for George S. Attard: ORCID iD orcid.org/0000-0001-8304-0742

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Date deposited: 24 Mar 2011 15:37
Last modified: 20 Jul 2019 01:21

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Contributors

Author: Richard J. Wilson
Author: Sarah R. Tyas
Author: Camilla F. Black
Author: Marcus K. Dymond

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