The University of Southampton
University of Southampton Institutional Repository

Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids

Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids
Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids
Recently, we reported that DNA associated with inverse hexagonal (HII) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase.(1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic HII phase throughout transcription. Here, we investigate the partitioning of DNA between an HII lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed HII liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the HII phase
0002-7863
9728-9732
Black, Camilla F.
17a9e6f0-03fe-4769-8455-c876f84316a1
Wilson, Richard J.
7f511c5c-11b7-455a-a1fb-1bc476be1a64
Nylander, Tommy
18d94ea0-6456-4eaf-b552-844261a37283
Dymond, Marcus K.
e180765b-039e-47ff-9841-ce6a5123a519
Attard, George S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36
Black, Camilla F.
17a9e6f0-03fe-4769-8455-c876f84316a1
Wilson, Richard J.
7f511c5c-11b7-455a-a1fb-1bc476be1a64
Nylander, Tommy
18d94ea0-6456-4eaf-b552-844261a37283
Dymond, Marcus K.
e180765b-039e-47ff-9841-ce6a5123a519
Attard, George S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36

Black, Camilla F., Wilson, Richard J., Nylander, Tommy, Dymond, Marcus K. and Attard, George S. (2010) Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids. Journal of the American Chemical Society, 132 (28), 9728-9732. (doi:10.1021/ja101550c). (PMID:20575567)

Record type: Article

Abstract

Recently, we reported that DNA associated with inverse hexagonal (HII) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase.(1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic HII phase throughout transcription. Here, we investigate the partitioning of DNA between an HII lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed HII liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the HII phase

Other
ja101550c - Version of Record
Download (1MB)

More information

e-pub ahead of print date: 24 June 2010
Published date: 21 July 2010
Organisations: Chemistry

Identifiers

Local EPrints ID: 178347
URI: https://eprints.soton.ac.uk/id/eprint/178347
ISSN: 0002-7863
PURE UUID: 37950e34-9843-43b8-9510-f08c90849ce0
ORCID for George S. Attard: ORCID iD orcid.org/0000-0001-8304-0742

Catalogue record

Date deposited: 24 Mar 2011 09:59
Last modified: 19 Jul 2019 01:17

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×