Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids
Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids
Recently, we reported that DNA associated with inverse hexagonal (HII) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase.(1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic HII phase throughout transcription. Here, we investigate the partitioning of DNA between an HII lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed HII liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the HII phase
9728-9732
Black, Camilla F.
17a9e6f0-03fe-4769-8455-c876f84316a1
Wilson, Richard J.
7f511c5c-11b7-455a-a1fb-1bc476be1a64
Nylander, Tommy
18d94ea0-6456-4eaf-b552-844261a37283
Dymond, Marcus K.
e180765b-039e-47ff-9841-ce6a5123a519
Attard, George S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36
21 July 2010
Black, Camilla F.
17a9e6f0-03fe-4769-8455-c876f84316a1
Wilson, Richard J.
7f511c5c-11b7-455a-a1fb-1bc476be1a64
Nylander, Tommy
18d94ea0-6456-4eaf-b552-844261a37283
Dymond, Marcus K.
e180765b-039e-47ff-9841-ce6a5123a519
Attard, George S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36
Black, Camilla F., Wilson, Richard J., Nylander, Tommy, Dymond, Marcus K. and Attard, George S.
(2010)
Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids.
Journal of the American Chemical Society, 132 (28), .
(doi:10.1021/ja101550c).
(PMID:20575567)
Abstract
Recently, we reported that DNA associated with inverse hexagonal (HII) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase.(1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic HII phase throughout transcription. Here, we investigate the partitioning of DNA between an HII lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed HII liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the HII phase
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ja101550c
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e-pub ahead of print date: 24 June 2010
Published date: 21 July 2010
Organisations:
Chemistry
Identifiers
Local EPrints ID: 178347
URI: http://eprints.soton.ac.uk/id/eprint/178347
ISSN: 0002-7863
PURE UUID: 37950e34-9843-43b8-9510-f08c90849ce0
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Date deposited: 24 Mar 2011 09:59
Last modified: 15 Mar 2024 02:45
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Contributors
Author:
Camilla F. Black
Author:
Richard J. Wilson
Author:
Tommy Nylander
Author:
Marcus K. Dymond
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