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Nucleotide requirements at positions +1 to +4 for the initiation of hepatitis C virus plus (+) strand RNA synthesis

Nucleotide requirements at positions +1 to +4 for the initiation of hepatitis C virus plus (+) strand RNA synthesis
Nucleotide requirements at positions +1 to +4 for the initiation of hepatitis C virus plus (+) strand RNA synthesis
RNA virus genome replication requires initiation at the precise terminus of template RNA. To investigate the nucleotide requirements for initiation of hepatitis C virus (HCV) positive strand RNA replication a hammerhead ribozyme was inserted at the 5' end of an HCV subgenomic replicon allowing the generation of replicons with all four possible nucleotides at position 1. This analysis revealed a preference for a purine nucleotide at this position for initiation of RNA replication. The sequence requirements at positions 2-4 in the context of the J6/JFH-1 virus were also examined by selecting replication competent virus from a pool containing randomised residues at these positions. There was a strong selection for both the wildtype C at position 2, and the wildtype sequence at positions 2-4 (CCU) . An A residue was well tolerated at positions 3 and 4, suggesting that efficient RNA replication is less dependent on these residues.
0022-1317
Nandasoma, Udvitha
9b2ce253-6bbb-41a2-90f0-6c13883ea9b3
McCormick, Chris
0fce14bf-2f67-4d08-991f-114dd1e7f0bd
Griffin, Stephen D.C.
3e357027-77ad-4b67-86e2-6d69f1ce3ed6
Harris, Mark
065415f3-96a7-443c-91b4-f75a5945f82b
Nandasoma, Udvitha
9b2ce253-6bbb-41a2-90f0-6c13883ea9b3
McCormick, Chris
0fce14bf-2f67-4d08-991f-114dd1e7f0bd
Griffin, Stephen D.C.
3e357027-77ad-4b67-86e2-6d69f1ce3ed6
Harris, Mark
065415f3-96a7-443c-91b4-f75a5945f82b

Nandasoma, Udvitha, McCormick, Chris, Griffin, Stephen D.C. and Harris, Mark (2011) Nucleotide requirements at positions +1 to +4 for the initiation of hepatitis C virus plus (+) strand RNA synthesis. Journal of General Virology. (doi:10.1099/vir.0.028423-0). (PMID:21270286)

Record type: Article

Abstract

RNA virus genome replication requires initiation at the precise terminus of template RNA. To investigate the nucleotide requirements for initiation of hepatitis C virus (HCV) positive strand RNA replication a hammerhead ribozyme was inserted at the 5' end of an HCV subgenomic replicon allowing the generation of replicons with all four possible nucleotides at position 1. This analysis revealed a preference for a purine nucleotide at this position for initiation of RNA replication. The sequence requirements at positions 2-4 in the context of the J6/JFH-1 virus were also examined by selecting replication competent virus from a pool containing randomised residues at these positions. There was a strong selection for both the wildtype C at position 2, and the wildtype sequence at positions 2-4 (CCU) . An A residue was well tolerated at positions 3 and 4, suggesting that efficient RNA replication is less dependent on these residues.

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More information

Published date: 26 January 2011

Identifiers

Local EPrints ID: 178771
URI: http://eprints.soton.ac.uk/id/eprint/178771
ISSN: 0022-1317
PURE UUID: 2b9a1e93-36f9-4e79-9b96-d0d1b26e9d27
ORCID for Chris McCormick: ORCID iD orcid.org/0000-0002-6155-9161

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Date deposited: 28 Mar 2011 10:51
Last modified: 14 Mar 2024 02:50

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Contributors

Author: Udvitha Nandasoma
Author: Chris McCormick ORCID iD
Author: Stephen D.C. Griffin
Author: Mark Harris

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