Biodegradable poly(alpha-hydroxy ester) blended microspheres as suitable carriers for retinal pigment epithelium cell transplantation
Biodegradable poly(alpha-hydroxy ester) blended microspheres as suitable carriers for retinal pigment epithelium cell transplantation
Five blends of poly(L-lactic acid) (PLLA) with poly(D,L-lactic-co-glycolic acid) (PLGA) were used to prepare microcarriers by a solvent evaporation technique. Microcarriers were evaluated as suitable scaffolds to facilitate retinal pigment epithelium (RPE) cell transplantation. The blend ratios were 90:10, 75:25, 50:50, 25:75, 10:90 (PLLA:PLGA). Samples of each microcarrier blend were coated with an extracellular matrix protein. Coated and uncoated microcarriers were seeded with a human RPE cell line. As the lactide unit content increased the size of microcarriers generally became larger and the surface more irregular. Cells remained proliferative and retained phenotype as confirmed by immunocytochemistry. Blends rich in PLLA were superior for maintenance of RPE cell viability. This study demonstrates for the first time the feasibility of using microcarriers as a vehicle for retinal cell transplantation for ocular disease.
transplantation, retinal pigment epithelium, poly(l-lactic acid), poly(d, l-lactic-co-glycolic acid), microcarriers
1233-1243
Thomson, H.A.
fdae50f5-d5c6-48da-a32b-6c64ed2cd8da
Treharne, A.J.
189aaa58-3159-4ed9-8927-3b133d9f6f9d
Backholer, L.S.
87910085-99e5-4879-81f8-8aa3a43dc2aa
Cuda, F.
8c738a55-70a8-4063-b063-bf83cdf0f270
Grossel, M.C.
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, A.J.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
15 December 2010
Thomson, H.A.
fdae50f5-d5c6-48da-a32b-6c64ed2cd8da
Treharne, A.J.
189aaa58-3159-4ed9-8927-3b133d9f6f9d
Backholer, L.S.
87910085-99e5-4879-81f8-8aa3a43dc2aa
Cuda, F.
8c738a55-70a8-4063-b063-bf83cdf0f270
Grossel, M.C.
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, A.J.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
Thomson, H.A., Treharne, A.J., Backholer, L.S., Cuda, F., Grossel, M.C. and Lotery, A.J.
(2010)
Biodegradable poly(alpha-hydroxy ester) blended microspheres as suitable carriers for retinal pigment epithelium cell transplantation.
Journal of Biomedical Materials Research Part A, 95 (4), .
(doi:10.1002/jbm.a.32940).
(PMID:20939049)
Abstract
Five blends of poly(L-lactic acid) (PLLA) with poly(D,L-lactic-co-glycolic acid) (PLGA) were used to prepare microcarriers by a solvent evaporation technique. Microcarriers were evaluated as suitable scaffolds to facilitate retinal pigment epithelium (RPE) cell transplantation. The blend ratios were 90:10, 75:25, 50:50, 25:75, 10:90 (PLLA:PLGA). Samples of each microcarrier blend were coated with an extracellular matrix protein. Coated and uncoated microcarriers were seeded with a human RPE cell line. As the lactide unit content increased the size of microcarriers generally became larger and the surface more irregular. Cells remained proliferative and retained phenotype as confirmed by immunocytochemistry. Blends rich in PLLA were superior for maintenance of RPE cell viability. This study demonstrates for the first time the feasibility of using microcarriers as a vehicle for retinal cell transplantation for ocular disease.
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Published date: 15 December 2010
Keywords:
transplantation, retinal pigment epithelium, poly(l-lactic acid), poly(d, l-lactic-co-glycolic acid), microcarriers
Identifiers
Local EPrints ID: 178857
URI: http://eprints.soton.ac.uk/id/eprint/178857
ISSN: 1549-3296
PURE UUID: 1c0b93b0-f118-489b-875b-1a88e503efe6
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Date deposited: 31 Mar 2011 10:58
Last modified: 15 Mar 2024 02:45
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Contributors
Author:
H.A. Thomson
Author:
A.J. Treharne
Author:
L.S. Backholer
Author:
F. Cuda
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