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New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes

New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes
New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes
The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with high efficiency (transligation). In an alternative strategy, intrastrand click ligation was employed to produce a functional hammerhead ribozyme containing a novel nucleic acid backbone mimic at the catalytic site (cis-ligation). The ability to synthesize long RNA strands by a combination of solid-phase synthesis and click ligation is an important addition to RNA chemistry. It is compatible with a plethora of site-specific modifications and is applicable to the synthesis of many biologically important RNA molecules.
chemical ligation, click rna ligation, cuaac, triazole backbone, biocompatible
0027-8424
15329-15334
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

El-Sagheer, Afaf H. and Brown, Tom (2010) New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes. Proceedings of the National Academy of Sciences, 107 (35), 15329-15334. (doi:10.1073/pnas.1006447107). (PMID:20713730)

Record type: Article

Abstract

The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with high efficiency (transligation). In an alternative strategy, intrastrand click ligation was employed to produce a functional hammerhead ribozyme containing a novel nucleic acid backbone mimic at the catalytic site (cis-ligation). The ability to synthesize long RNA strands by a combination of solid-phase synthesis and click ligation is an important addition to RNA chemistry. It is compatible with a plethora of site-specific modifications and is applicable to the synthesis of many biologically important RNA molecules.

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Published date: 31 July 2010
Keywords: chemical ligation, click rna ligation, cuaac, triazole backbone, biocompatible
Organisations: Chemistry

Identifiers

Local EPrints ID: 178909
URI: http://eprints.soton.ac.uk/id/eprint/178909
ISSN: 0027-8424
PURE UUID: e9052a4d-8d41-49b8-b1ef-bf7b56b2502d

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Date deposited: 31 Mar 2011 10:27
Last modified: 25 Nov 2019 21:16

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