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Nanomolar detection with high sensitivity microfluidic absorption cells manufactured in tinted PMMA for chemical analysis

Nanomolar detection with high sensitivity microfluidic absorption cells manufactured in tinted PMMA for chemical analysis
Nanomolar detection with high sensitivity microfluidic absorption cells manufactured in tinted PMMA for chemical analysis
We describe a novel, cost effective and simple technique for the manufacture of high sensitivity absorption cells for microfluidic analytical systems. The cells are made from tinted polymethyl methacrylate (PMMA) in which microfluidic channels are fabricated. Two windows (typically 250 ?m thick, resulting in little optical power loss) are formed at either end of the channel through which light is coupled. Unwanted stray light from the emitter passes through a greater thickness of the tinted substrate (typically the length of the cell) and is preferentially absorbed. In effect, this creates a pin-hole configuration over the length of the absorption cell, providing improved performances (sensitivity, S/N ratios, baseline noise and limit of detection) when used as an absorption cell compared to clear substrates. The method is used to achieve a LOD of 20 nM with a colourimetric iron assay and a LOD of 0.22 milli-absorption units with a pH assay.
Microfluidic, PMMA, Colourimetric assays, Absorption, Microsensors
0039-9140
235-239
Floquet, Cedric F.A.
9e0adc5e-10f1-4d67-81cc-63157949b704
Sieben, Vincent J.
7c2fe2a3-4b9c-4359-835e-ea375b25d30f
Milani, Ambra
839ef5af-ea55-4f7c-a5d6-5dfa4c5b62aa
Joly, E
76a498ab-2ec9-4dda-a3d7-50b7f2bbdb7a
Ogilvie, Iain R.G.
fe473057-bf44-46d1-8add-88060037beb5
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Floquet, Cedric F.A.
9e0adc5e-10f1-4d67-81cc-63157949b704
Sieben, Vincent J.
7c2fe2a3-4b9c-4359-835e-ea375b25d30f
Milani, Ambra
839ef5af-ea55-4f7c-a5d6-5dfa4c5b62aa
Joly, E
76a498ab-2ec9-4dda-a3d7-50b7f2bbdb7a
Ogilvie, Iain R.G.
fe473057-bf44-46d1-8add-88060037beb5
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f

Floquet, Cedric F.A., Sieben, Vincent J., Milani, Ambra, Joly, E, Ogilvie, Iain R.G., Morgan, Hywel and Mowlem, Matthew C. (2011) Nanomolar detection with high sensitivity microfluidic absorption cells manufactured in tinted PMMA for chemical analysis. Talanta, 84 (1), 235-239. (doi:10.1016/j.talanta.2010.12.026).

Record type: Article

Abstract

We describe a novel, cost effective and simple technique for the manufacture of high sensitivity absorption cells for microfluidic analytical systems. The cells are made from tinted polymethyl methacrylate (PMMA) in which microfluidic channels are fabricated. Two windows (typically 250 ?m thick, resulting in little optical power loss) are formed at either end of the channel through which light is coupled. Unwanted stray light from the emitter passes through a greater thickness of the tinted substrate (typically the length of the cell) and is preferentially absorbed. In effect, this creates a pin-hole configuration over the length of the absorption cell, providing improved performances (sensitivity, S/N ratios, baseline noise and limit of detection) when used as an absorption cell compared to clear substrates. The method is used to achieve a LOD of 20 nM with a colourimetric iron assay and a LOD of 0.22 milli-absorption units with a pH assay.

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More information

Published date: 1 March 2011
Keywords: Microfluidic, PMMA, Colourimetric assays, Absorption, Microsensors
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 179503
URI: http://eprints.soton.ac.uk/id/eprint/179503
ISSN: 0039-9140
PURE UUID: f22e6db8-b595-44b1-a1bd-969d9f764953
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Matthew C. Mowlem: ORCID iD orcid.org/0000-0001-7613-6121

Catalogue record

Date deposited: 01 Apr 2011 13:01
Last modified: 15 Mar 2024 03:18

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Contributors

Author: Cedric F.A. Floquet
Author: Vincent J. Sieben
Author: Ambra Milani
Author: E Joly
Author: Iain R.G. Ogilvie
Author: Hywel Morgan ORCID iD
Author: Matthew C. Mowlem ORCID iD

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