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Optical propulsion of mammalian eukaryotic cells on an integrated channel waveguide

Optical propulsion of mammalian eukaryotic cells on an integrated channel waveguide
Optical propulsion of mammalian eukaryotic cells on an integrated channel waveguide
The optical propulsion of mammalian eukaryotic cells along the surface of an integrated channel waveguide is demonstrated. 10µm diameter polymethylmethacrylate (PMMA) spherical particles and similarly sized mammalian eukaryotic cells in aqueous medium are deposited in a reservoir over a caesium ion-exchanged channel waveguide. Light from a fibre laser at 1064nm was coupled into the waveguide, causing the polymer particles or cells to be propelled along the waveguide at a velocity which is dependent upon the laser power. A theoretical model was used to predict the propulsion velocity as a function of the refractive index of the particle. The experimental results obtained for the PMMA particles and the mammalian cells show that for input powers greater than 50mW the propulsion velocity is approximately that obtained by the theoretical model. For input powers of less than ~50mW neither particles nor cells were propelled; this is considered to be a result of surface forces (which are not considered in the theoretical model). The results are discussed in light of the potential application of optical channel waveguides for bioanalytical applications, namely in the identification and sorting of mammalian cells from mixed populations without the need for fluorescence or antibody labels.
9780819484666
Mohamad Shahimin, M.
991cc764-7645-464d-92da-a3492ca15517
Perney, N.M.B.
169bd490-31cb-4379-b25b-ae3e7880081b
Brooks, Suzanne E.
4b0ecd34-a592-46b3-a21f-f83bdda46c7b
Hanley, N.
f64325bc-b0ed-4249-8f11-dbe4cdcbff93
Wright, K.L.
4478f039-bdce-4aff-ac60-8dd704f4e2e4
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Becker, Holger
971bcd08-babd-420b-8b42-4ae30867503f
Gray, Bonnie L.
47a88cdd-6516-4740-999e-470770467d2b
Mohamad Shahimin, M.
991cc764-7645-464d-92da-a3492ca15517
Perney, N.M.B.
169bd490-31cb-4379-b25b-ae3e7880081b
Brooks, Suzanne E.
4b0ecd34-a592-46b3-a21f-f83bdda46c7b
Hanley, N.
f64325bc-b0ed-4249-8f11-dbe4cdcbff93
Wright, K.L.
4478f039-bdce-4aff-ac60-8dd704f4e2e4
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Becker, Holger
971bcd08-babd-420b-8b42-4ae30867503f
Gray, Bonnie L.
47a88cdd-6516-4740-999e-470770467d2b

Mohamad Shahimin, M., Perney, N.M.B., Brooks, Suzanne E., Hanley, N., Wright, K.L., Wilkinson, J.S. and Melvin, T. , Becker, Holger and Gray, Bonnie L. (eds.) (2011) Optical propulsion of mammalian eukaryotic cells on an integrated channel waveguide. Proceedings of SPIE, 7929 (792909). (doi:10.1117/12.874019).

Record type: Article

Abstract

The optical propulsion of mammalian eukaryotic cells along the surface of an integrated channel waveguide is demonstrated. 10µm diameter polymethylmethacrylate (PMMA) spherical particles and similarly sized mammalian eukaryotic cells in aqueous medium are deposited in a reservoir over a caesium ion-exchanged channel waveguide. Light from a fibre laser at 1064nm was coupled into the waveguide, causing the polymer particles or cells to be propelled along the waveguide at a velocity which is dependent upon the laser power. A theoretical model was used to predict the propulsion velocity as a function of the refractive index of the particle. The experimental results obtained for the PMMA particles and the mammalian cells show that for input powers greater than 50mW the propulsion velocity is approximately that obtained by the theoretical model. For input powers of less than ~50mW neither particles nor cells were propelled; this is considered to be a result of surface forces (which are not considered in the theoretical model). The results are discussed in light of the potential application of optical channel waveguides for bioanalytical applications, namely in the identification and sorting of mammalian cells from mixed populations without the need for fluorescence or antibody labels.

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More information

Published date: 23 January 2011
Additional Information: Funded by BBSRC: Optical selection of stem cells: application to human embryonic germ cells (BB/D014670/2)
Organisations: Optoelectronics Research Centre, Electronics & Computer Science, Medicine, Biological Sciences Research

Identifiers

Local EPrints ID: 179815
URI: http://eprints.soton.ac.uk/id/eprint/179815
ISBN: 9780819484666
PURE UUID: e49b8d90-1f2e-4521-a848-e68361f94e83
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 07 Apr 2011 13:26
Last modified: 07 Oct 2020 02:11

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Contributors

Author: M. Mohamad Shahimin
Author: N.M.B. Perney
Author: Suzanne E. Brooks
Author: N. Hanley
Author: K.L. Wright
Author: J.S. Wilkinson ORCID iD
Author: T. Melvin
Editor: Holger Becker
Editor: Bonnie L. Gray

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