Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis
Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis
Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic science, point-of-care diagnostics and detection of bioterrorism agents. Despite its broad utility, the search for new tests continues, inspired by second and third generation DNA sequencing technologies and fuelled by progress in single molecule fluorescence spectroscopy, nanotechnology and microfabrication. These new methods promise the direct detection of nucleic acids without the need for enzymatic amplification. In this feature article, we provide a chemist's perspective on this multidisciplinary area, introducing the concepts of single molecule detection then focussing on the selection of labels and probe chemistry suitable for generating a signal detectable by ultrasensitive fluorescence spectroscopy. Finally, we discuss the further developments that are required to incorporate these detection platforms into integrated ‘sample-in-answer-out’ instruments, capable of detecting many target sequences in a matter of minutes
3717-3735
Ranasinghe, Rohan T.
b29fc8b4-2a66-430a-85fa-ff1c9c261f32
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
2011
Ranasinghe, Rohan T.
b29fc8b4-2a66-430a-85fa-ff1c9c261f32
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Ranasinghe, Rohan T. and Brown, Tom
(2011)
Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis.
Chemical Communications, 47 (13), .
(doi:10.1039/c0cc04215c).
Abstract
Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic science, point-of-care diagnostics and detection of bioterrorism agents. Despite its broad utility, the search for new tests continues, inspired by second and third generation DNA sequencing technologies and fuelled by progress in single molecule fluorescence spectroscopy, nanotechnology and microfabrication. These new methods promise the direct detection of nucleic acids without the need for enzymatic amplification. In this feature article, we provide a chemist's perspective on this multidisciplinary area, introducing the concepts of single molecule detection then focussing on the selection of labels and probe chemistry suitable for generating a signal detectable by ultrasensitive fluorescence spectroscopy. Finally, we discuss the further developments that are required to incorporate these detection platforms into integrated ‘sample-in-answer-out’ instruments, capable of detecting many target sequences in a matter of minutes
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ultrasensitive_fluorescent_methods_chem_comm_2011.pdf
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Published date: 2011
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Local EPrints ID: 179817
URI: http://eprints.soton.ac.uk/id/eprint/179817
ISSN: 1359-7345
PURE UUID: 59fe6976-f2fe-43c3-9c74-083da650dfda
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Date deposited: 04 Apr 2011 14:01
Last modified: 14 Mar 2024 02:50
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Author:
Rohan T. Ranasinghe
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