The University of Southampton
University of Southampton Institutional Repository

Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis

Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis
Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis
Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic science, point-of-care diagnostics and detection of bioterrorism agents. Despite its broad utility, the search for new tests continues, inspired by second and third generation DNA sequencing technologies and fuelled by progress in single molecule fluorescence spectroscopy, nanotechnology and microfabrication. These new methods promise the direct detection of nucleic acids without the need for enzymatic amplification. In this feature article, we provide a chemist's perspective on this multidisciplinary area, introducing the concepts of single molecule detection then focussing on the selection of labels and probe chemistry suitable for generating a signal detectable by ultrasensitive fluorescence spectroscopy. Finally, we discuss the further developments that are required to incorporate these detection platforms into integrated ‘sample-in-answer-out’ instruments, capable of detecting many target sequences in a matter of minutes
1359-7345
3717-3735
Ranasinghe, Rohan T.
b29fc8b4-2a66-430a-85fa-ff1c9c261f32
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Ranasinghe, Rohan T.
b29fc8b4-2a66-430a-85fa-ff1c9c261f32
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Ranasinghe, Rohan T. and Brown, Tom (2011) Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis. Chemical Communications, 47 (13), 3717-3735. (doi:10.1039/c0cc04215c).

Record type: Article

Abstract

Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic science, point-of-care diagnostics and detection of bioterrorism agents. Despite its broad utility, the search for new tests continues, inspired by second and third generation DNA sequencing technologies and fuelled by progress in single molecule fluorescence spectroscopy, nanotechnology and microfabrication. These new methods promise the direct detection of nucleic acids without the need for enzymatic amplification. In this feature article, we provide a chemist's perspective on this multidisciplinary area, introducing the concepts of single molecule detection then focussing on the selection of labels and probe chemistry suitable for generating a signal detectable by ultrasensitive fluorescence spectroscopy. Finally, we discuss the further developments that are required to incorporate these detection platforms into integrated ‘sample-in-answer-out’ instruments, capable of detecting many target sequences in a matter of minutes

Text
ultrasensitive_fluorescent_methods_chem_comm_2011.pdf - Version of Record
Download (5MB)

More information

Published date: 2011

Identifiers

Local EPrints ID: 179817
URI: http://eprints.soton.ac.uk/id/eprint/179817
ISSN: 1359-7345
PURE UUID: 59fe6976-f2fe-43c3-9c74-083da650dfda

Catalogue record

Date deposited: 04 Apr 2011 14:01
Last modified: 14 Mar 2024 02:50

Export record

Altmetrics

Contributors

Author: Rohan T. Ranasinghe
Author: Tom Brown

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×