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Cloning and expression throughout mouse development of mfat, a homologue of the Drosophila tumour suppressor gene fat

Cloning and expression throughout mouse development of mfat, a homologue of the Drosophila tumour suppressor gene fat
Cloning and expression throughout mouse development of mfat, a homologue of the Drosophila tumour suppressor gene fat
We present the entire sequence of the mouse Fat orthologue (mFat1), a protein of 4,588 amino acids with 34 cadherin repeats, 27 potential N-glycosylation sites, five EGF repeats and a laminin A G-motif in its extracellular domain. A single transmembrane region is followed by a cytoplasmic domain containing putative catenin-binding sequences. mFat1 shows high homology to human FAT and lesser homology to Drosophila Fat. The sequence of this giant cadherin suggests that it is unlikely to have a homophilic adhesive function, but may mediate heterophilic adhesion or play a signalling role. Expression analysis shows that the mfat1 gene is expressed early in pre-implantation mouse development, at the compact eight cell stage. Whole-mount and section in situ analyses show that transcripts are widely expressed throughout post-implantation development, most notably in the limb buds, branchial arches, forming somites, and in particular in the proliferating ventricular zones in the brain, being down-regulated as cells cease dividing. RT-PCR detects widespread expression in the adult suggesting a role in proliferation and differentiation of many tissues and cell types.
cadherin, tumour suppressor, egf, catenins
1058-8388
233-240
Cox, Barnaby
a9928a3a-b5bd-4b60-b343-5e16e6ccf140
Hadjantonakis, Anna-Katerina
10360392-d8bb-4559-aacd-bde9a40f71fc
Collins, Jane E.
be0e66f1-3036-47fa-9d7e-914c48710ba4
Magee, Anthony I.
5bff1fec-47d6-427d-9bc4-3c2a2c570d53
Cox, Barnaby
a9928a3a-b5bd-4b60-b343-5e16e6ccf140
Hadjantonakis, Anna-Katerina
10360392-d8bb-4559-aacd-bde9a40f71fc
Collins, Jane E.
be0e66f1-3036-47fa-9d7e-914c48710ba4
Magee, Anthony I.
5bff1fec-47d6-427d-9bc4-3c2a2c570d53

Cox, Barnaby, Hadjantonakis, Anna-Katerina, Collins, Jane E. and Magee, Anthony I. (2000) Cloning and expression throughout mouse development of mfat, a homologue of the Drosophila tumour suppressor gene fat. Developmental Dynamics, 217 (3), 233-240. (doi:10.1002/(ISSN)1097-0177).

Record type: Article

Abstract

We present the entire sequence of the mouse Fat orthologue (mFat1), a protein of 4,588 amino acids with 34 cadherin repeats, 27 potential N-glycosylation sites, five EGF repeats and a laminin A G-motif in its extracellular domain. A single transmembrane region is followed by a cytoplasmic domain containing putative catenin-binding sequences. mFat1 shows high homology to human FAT and lesser homology to Drosophila Fat. The sequence of this giant cadherin suggests that it is unlikely to have a homophilic adhesive function, but may mediate heterophilic adhesion or play a signalling role. Expression analysis shows that the mfat1 gene is expressed early in pre-implantation mouse development, at the compact eight cell stage. Whole-mount and section in situ analyses show that transcripts are widely expressed throughout post-implantation development, most notably in the limb buds, branchial arches, forming somites, and in particular in the proliferating ventricular zones in the brain, being down-regulated as cells cease dividing. RT-PCR detects widespread expression in the adult suggesting a role in proliferation and differentiation of many tissues and cell types.

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More information

Published date: March 2000
Keywords: cadherin, tumour suppressor, egf, catenins

Identifiers

Local EPrints ID: 179855
URI: http://eprints.soton.ac.uk/id/eprint/179855
ISSN: 1058-8388
PURE UUID: 774b8867-b6e2-4325-9a1d-aff5d37de326

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Date deposited: 05 Apr 2011 13:20
Last modified: 19 Jun 2020 16:37

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