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The effect of a selective octopamine antagonist, epinastine, on pharyngeal pumping in Caenorhabditis elegans

The effect of a selective octopamine antagonist, epinastine, on pharyngeal pumping in Caenorhabditis elegans
The effect of a selective octopamine antagonist, epinastine, on pharyngeal pumping in Caenorhabditis elegans
This paper investigates the effect of epinastine, a selective octopamine antagonist in invertebrates, in Caenorhabditis elegans. Specifically, its ability to block the inhibitory action of octopamine on C. elegans-isolated pharynx was assayed. Isolated pharynxes were stimulated to pump by the addition of 500 nM 5-hydroxytryptamine (5-HT) (113 ± 2 per 30 s, n = 15). Octopamine inhibited the 5-HT-induced pumping in a concentration-dependent manner (threshold 1-5 ?M) with a 61 ± 11% inhibition with 50 ?M (n = 5). Epinastine (0.1 ?M) antagonized the inhibitory response to octopamine (P < 0.001; n = 15). Tyramine also inhibited pharyngeal pumping induced by 5-HT but was less potent than octopamine. Tyramine, 50 ?M to 1 mM, gave a transient inhibition e.g. of 40 ± 5% at 50 ?M (n = 5). A higher (10 ?M) concentration of epinastine was required to block the tryamine response compared with octopamine. It is concluded that epinastine selectively antagonizes the effect of octopamine on C. elegans pharynx. Further studies are required to test its selectivity for octopamine in other tissues and other nematodes
1354-2516
47-52
Packham, Rachel
12cf38a8-75bc-4845-b567-9c11692ecfb5
Walker, Robert J.
9368ac2d-f1e9-4bd9-a4b4-4a161c4aa140
Holden-Dye, Lindy
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Packham, Rachel
12cf38a8-75bc-4845-b567-9c11692ecfb5
Walker, Robert J.
9368ac2d-f1e9-4bd9-a4b4-4a161c4aa140
Holden-Dye, Lindy
8032bf60-5db6-40cb-b71c-ddda9d212c8e

Packham, Rachel, Walker, Robert J. and Holden-Dye, Lindy (2010) The effect of a selective octopamine antagonist, epinastine, on pharyngeal pumping in Caenorhabditis elegans. Invertebrate Neuroscience, 10 (1), 47-52. (doi:10.1007/s10158-010-0107-9). (PMID:20967561)

Record type: Article

Abstract

This paper investigates the effect of epinastine, a selective octopamine antagonist in invertebrates, in Caenorhabditis elegans. Specifically, its ability to block the inhibitory action of octopamine on C. elegans-isolated pharynx was assayed. Isolated pharynxes were stimulated to pump by the addition of 500 nM 5-hydroxytryptamine (5-HT) (113 ± 2 per 30 s, n = 15). Octopamine inhibited the 5-HT-induced pumping in a concentration-dependent manner (threshold 1-5 ?M) with a 61 ± 11% inhibition with 50 ?M (n = 5). Epinastine (0.1 ?M) antagonized the inhibitory response to octopamine (P < 0.001; n = 15). Tyramine also inhibited pharyngeal pumping induced by 5-HT but was less potent than octopamine. Tyramine, 50 ?M to 1 mM, gave a transient inhibition e.g. of 40 ± 5% at 50 ?M (n = 5). A higher (10 ?M) concentration of epinastine was required to block the tryamine response compared with octopamine. It is concluded that epinastine selectively antagonizes the effect of octopamine on C. elegans pharynx. Further studies are required to test its selectivity for octopamine in other tissues and other nematodes

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Published date: December 2010

Identifiers

Local EPrints ID: 180029
URI: http://eprints.soton.ac.uk/id/eprint/180029
ISSN: 1354-2516
PURE UUID: c2d30c61-3216-4854-90dc-ba8391343e2c
ORCID for Robert J. Walker: ORCID iD orcid.org/0000-0002-9031-7671
ORCID for Lindy Holden-Dye: ORCID iD orcid.org/0000-0002-9704-1217

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Date deposited: 06 Apr 2011 14:23
Last modified: 18 Feb 2021 17:37

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