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The effect of a selective octopamine antagonist, epinastine, on pharyngeal pumping in Caenorhabditis elegans

Packham, Rachel, Walker, Robert J. and Holden-Dye, Lindy (2010) The effect of a selective octopamine antagonist, epinastine, on pharyngeal pumping in Caenorhabditis elegans Invertebrate Neuroscience, 10, (1), pp. 47-52. (doi:10.1007/s10158-010-0107-9). (PMID:20967561).

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This paper investigates the effect of epinastine, a selective octopamine antagonist in invertebrates, in Caenorhabditis elegans. Specifically, its ability to block the inhibitory action of octopamine on C. elegans-isolated pharynx was assayed. Isolated pharynxes were stimulated to pump by the addition of 500 nM 5-hydroxytryptamine (5-HT) (113 ± 2 per 30 s, n = 15). Octopamine inhibited the 5-HT-induced pumping in a concentration-dependent manner (threshold 1-5 ?M) with a 61 ± 11% inhibition with 50 ?M (n = 5). Epinastine (0.1 ?M) antagonized the inhibitory response to octopamine (P < 0.001; n = 15). Tyramine also inhibited pharyngeal pumping induced by 5-HT but was less potent than octopamine. Tyramine, 50 ?M to 1 mM, gave a transient inhibition e.g. of 40 ± 5% at 50 ?M (n = 5). A higher (10 ?M) concentration of epinastine was required to block the tryamine response compared with octopamine. It is concluded that epinastine selectively antagonizes the effect of octopamine on C. elegans pharynx. Further studies are required to test its selectivity for octopamine in other tissues and other nematodes

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Published date: December 2010


Local EPrints ID: 180029
ISSN: 1354-2516
PURE UUID: c2d30c61-3216-4854-90dc-ba8391343e2c

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Date deposited: 06 Apr 2011 14:23
Last modified: 18 Jul 2017 12:01

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Author: Rachel Packham
Author: Robert J. Walker

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