The University of Southampton
University of Southampton Institutional Repository

Selective toxicity of the anthelmintic emodepside revealed by heterologous expression of human KCNMA1 in caenorhabditis elegans

Record type: Article

Emodepside is a resistance-breaking anthelmintic of a new chemical class, the cyclooctadepsipeptides. A major determinant of its anthelmintic effect is the calcium-activated potassium channel, SLO-1. SLO-1 belongs to a family of channels that are highly conserved across the animal phyla and regulate neurosecretion, hormone release, muscle contraction and neuronal network excitability. To investigate the selective toxicity of emodepside we performed transgenic experiments in which the nematode SLO-1 channel was swapped for a mammalian orthologue, human KCNMA1. Expression of either the human channel or C. elegans slo-1 from the native slo-1 promoter in a C. elegans slo- functional null rescued behavioural deficits that otherwise resulted from loss of slo-1 signalling. However, worms expressing the human channel were 10 to 100-fold less sensitive to emodepside than those expressing the nematode channel. Strains expressing the human KCNMA1 channel were preferentially sensitive to the mammalian channel agonists NS1619 and rottlerin. In the C. elegans pharyngeal nervous system slo-1 is expressed in neurones not muscle and cell specific rescue experiments have previously shown that emodepside inhibits serotonin-stimulated feeding by interfering with SLO-1 signalling in the nervous system. Here we show that ectopic over-expression of slo-1 in pharyngeal muscle confers sensitivity of the muscle to emodepside, consistent with a direct interaction of emodepside with the channel. Taken together these data predict an emodepside selective pharmacophore harboured by SLO-1. This has implications for the development of this drug/target interface for the treatment of helminth infections

PDF http___submit-molpharm.aspetjournals.pdf - Other
Restricted to Repository staff only
Download (6MB)

Citation

Crisford, Anna, Murray, Caitriona, O'Connor, Vincent, Edwards, Richard J., Kruger, Nina, Welz, Claudia, von Samson-Himmelstjerna, Georg, Harder, Achim, Walker, Robert J. and Holden-Dye, Lindy (2011) Selective toxicity of the anthelmintic emodepside revealed by heterologous expression of human KCNMA1 in caenorhabditis elegans Molecular Pharmacology, 79, (6), pp. 1031-1043. (doi:10.1124/mol.111.071043). (PMID:21415309).

More information

Published date: 2011
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 180031
URI: http://eprints.soton.ac.uk/id/eprint/180031
ISSN: 0026-895X
PURE UUID: 50bdfd2e-7e0f-42b9-9a2c-4a39dc81736e

Catalogue record

Date deposited: 06 Apr 2011 14:16
Last modified: 18 Jul 2017 12:01

Export record

Altmetrics

Contributors

Author: Anna Crisford
Author: Caitriona Murray
Author: Vincent O'Connor
Author: Richard J. Edwards
Author: Nina Kruger
Author: Claudia Welz
Author: Georg von Samson-Himmelstjerna
Author: Achim Harder
Author: Robert J. Walker

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×