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Facilitated transporters mediate net efflux of amino acids to the fetus across the basal membrane of the placental syncytiotrophoblast

Record type: Article

Fetal growth depends on placental transfer of amino acids from maternal to fetal blood. The mechanisms of net amino acid efflux across the basal membrane (BM) of the placental syncytiotrophoblast to the fetus, although vital for amino acid transport, are poorly understood. We examined the hypothesis that facilitated diffusion by the amino acid transporters TAT1, LAT3 and LAT4 plays an important role in this process, with possible effects on fetal growth. Methods: Amino acid transfer was measured in isolated perfused human placental cotyledons (n=5/experiment) using techniques which distinguish between different transport processes. Placental TAT1, LAT3 and LAT4 proteins were measured, and mRNA expression levels (measured using real-time quantitative-PCR) were related to fetal and neonatal anthropometry and dual-energy X-ray absorptiometry measurements of neonatal lean mass in 102 Southampton Women's Survey (SWS) infants. Results: Under conditions preventing transport by amino acid exchangers, all amino acids appearing in the fetal circulation were substrates of TAT1, LAT3 or LAT4. Western blots demonstrated the presence of TAT1, LAT3 and LAT4 in placental BM preparations. Placental TAT1 and LAT3 mRNA expression were positively associated with measures of fetal growth in SWS infants (P<0.05). Conclusions: We provide evidence that the efflux transporters TAT1, LAT3 and LAT4 are present in the human placental BM, and may play an important role in the net efflux of amino acids to the fetus. Unlike other transporters they can increase fetal amino acid concentrations. Consistent with a role in placental amino acid transfer capacity and fetal growth TAT1 and LAT3 mRNA expression showed positive associations with infant size at birth.

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Citation

Cleal, J. K., Glazier, J. D., Ntani, G., Crozier, S. R., Day, P. E., Harvey, N. C., Robinson, S. M., Cooper, C., Godfrey, K. M., Hanson, M. A. and Lewis, R. M. (2011) Facilitated transporters mediate net efflux of amino acids to the fetus across the basal membrane of the placental syncytiotrophoblast The Journal of Physiology, 589, (4), pp. 987-997. (doi:10.1113/jphysiol.2010.198549). (PMID:21224231).

More information

Published date: 4 January 2011
Keywords: MVM, maternal-facing microvillous plasma membrane, BM, basal plasma membrane
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 181017
URI: http://eprints.soton.ac.uk/id/eprint/181017
ISSN: 0022-3751
PURE UUID: 4f385277-e336-4222-bf53-98fb81d15ac2
ORCID for S. M. Robinson: ORCID iD orcid.org/0000-0003-1766-7269
ORCID for K. M. Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for R. M. Lewis: ORCID iD orcid.org/0000-0003-4044-9104

Catalogue record

Date deposited: 13 Apr 2011 11:49
Last modified: 18 Jul 2017 12:00

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Contributors

Author: J. K. Cleal
Author: J. D. Glazier
Author: G. Ntani
Author: S. R. Crozier
Author: P. E. Day
Author: N. C. Harvey
Author: S. M. Robinson ORCID iD
Author: C. Cooper
Author: K. M. Godfrey ORCID iD
Author: M. A. Hanson
Author: R. M. Lewis ORCID iD

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