HeLa cell transfection using a novel sonoporation system
HeLa cell transfection using a novel sonoporation system
Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, p < 0.05) at the resonant frequency of 980 kHz at 100 Vp-p (19.5 MPa) with a cell viability of 77% after 10 s of insonication.
HeLa cell and pEGFP-N1, sonoporation, ultrasonic standing wave
927-934
Rodamporn, S.
68a5c7d8-df55-482d-a200-8b56a694cd35
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Beeby, S.P.
ba565001-2812-4300-89f1-fe5a437ecb0d
Boltryk, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Sanchez-Elsner, T.
b8799f8d-e2b4-4b37-b77c-f2f0e8e2070d
April 2011
Rodamporn, S.
68a5c7d8-df55-482d-a200-8b56a694cd35
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Beeby, S.P.
ba565001-2812-4300-89f1-fe5a437ecb0d
Boltryk, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Sanchez-Elsner, T.
b8799f8d-e2b4-4b37-b77c-f2f0e8e2070d
Rodamporn, S., Harris, N.R., Beeby, S.P., Boltryk, R.J. and Sanchez-Elsner, T.
(2011)
HeLa cell transfection using a novel sonoporation system.
IEEE Transactions on Biomedical Engineering, 58 (4), .
(doi:10.1109/TBME.2010.2089521).
(PMID:20977982)
Abstract
Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, p < 0.05) at the resonant frequency of 980 kHz at 100 Vp-p (19.5 MPa) with a cell viability of 77% after 10 s of insonication.
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e-pub ahead of print date: 25 October 2010
Published date: April 2011
Keywords:
HeLa cell and pEGFP-N1, sonoporation, ultrasonic standing wave
Organisations:
Electronics & Computer Science, Infection Inflammation & Immunity, Engineering Science Unit
Identifiers
Local EPrints ID: 181161
URI: http://eprints.soton.ac.uk/id/eprint/181161
ISSN: 0018-9294
PURE UUID: 24ac7ecf-c6bf-4a79-849e-8c3893b4d26f
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Date deposited: 14 Apr 2011 09:16
Last modified: 07 Dec 2024 02:43
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Contributors
Author:
S. Rodamporn
Author:
N.R. Harris
Author:
S.P. Beeby
Author:
R.J. Boltryk
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