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HeLa cell transfection using a novel sonoporation system

HeLa cell transfection using a novel sonoporation system
HeLa cell transfection using a novel sonoporation system
Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, <formula formulatype="inline"><tex Notation="TeX">$p$</tex></formula> < 0.05) at the resonant frequency of 980 kHz at 100 V<formula formulatype="inline"><tex Notation="TeX">$_{\rm{p-p}}$</tex></formula> (19.5 MPa) with a cell viability of 77% after 10 s of insonication.
HeLa cell and pEGFP-N1, sonoporation, ultrasonic standing wave
0018-9294
927-934
Rodamporn, S.
68a5c7d8-df55-482d-a200-8b56a694cd35
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Beeby, S.P.
ba565001-2812-4300-89f1-fe5a437ecb0d
Boltryk, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Sanchez-Elsner, T.
b8799f8d-e2b4-4b37-b77c-f2f0e8e2070d
Rodamporn, S.
68a5c7d8-df55-482d-a200-8b56a694cd35
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Beeby, S.P.
ba565001-2812-4300-89f1-fe5a437ecb0d
Boltryk, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Sanchez-Elsner, T.
b8799f8d-e2b4-4b37-b77c-f2f0e8e2070d

Rodamporn, S., Harris, N.R., Beeby, S.P., Boltryk, R.J. and Sanchez-Elsner, T. (2011) HeLa cell transfection using a novel sonoporation system IEEE Transactions on Biomedical Engineering, 58, (4), pp. 927-934. (doi:10.1109/TBME.2010.2089521). (PMID:20977982).

Record type: Article

Abstract

Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, <formula formulatype="inline"><tex Notation="TeX">$p$</tex></formula> < 0.05) at the resonant frequency of 980 kHz at 100 V<formula formulatype="inline"><tex Notation="TeX">$_{\rm{p-p}}$</tex></formula> (19.5 MPa) with a cell viability of 77% after 10 s of insonication.

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More information

e-pub ahead of print date: 25 October 2010
Published date: April 2011
Keywords: HeLa cell and pEGFP-N1, sonoporation, ultrasonic standing wave
Organisations: Electronics & Computer Science, Infection Inflammation & Immunity, Engineering Science Unit

Identifiers

Local EPrints ID: 181161
URI: http://eprints.soton.ac.uk/id/eprint/181161
ISSN: 0018-9294
PURE UUID: 24ac7ecf-c6bf-4a79-849e-8c3893b4d26f
ORCID for N.R. Harris: ORCID iD orcid.org/0000-0003-4122-2219
ORCID for S.P. Beeby: ORCID iD orcid.org/0000-0002-0800-1759

Catalogue record

Date deposited: 14 Apr 2011 09:16
Last modified: 18 Jul 2017 11:59

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Contributors

Author: S. Rodamporn
Author: N.R. Harris ORCID iD
Author: S.P. Beeby ORCID iD
Author: R.J. Boltryk

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