Increased conjunctival expression of protease activated receptor 2 (PAR-2) in seasonal allergic conjunctivitis: a role for abnormal conjunctival epithelial permeability in disease pathogenesis?
Increased conjunctival expression of protease activated receptor 2 (PAR-2) in seasonal allergic conjunctivitis: a role for abnormal conjunctival epithelial permeability in disease pathogenesis?
Purpose: aeroallergen exposure to the conjunctival epithelium in seasonal allergic conjunctivitis (SAC) may induce a cellular stress response that disrupts the barrier properties of the conjunctival epithelium resulting in allergic disease. Whether such changes occur in SAC is not known. Epithelial permeability is known to be increased when protease activated receptor 2 (PAR-2) is activated. We evaluated the expression of PAR-2 in patients with SAC in season (SACS) and compared them to control non-atopic subjects or those with out of season allergic conjunctivitis (OSAC).
Methods: 6 SACS, 8 normal and 4 OSAC specimens were examined immunohistochemically for PAR-2 and quantified in a masked fashion for the percentage of epithelia stained for each marker using Image-J software. Conjunctival epithelial heights were measured in all groups to confirm the presence of allergic eye disease.
Results: mean percentage staining of PAR-2 was significantly greater in SACS when compared to normals (73.4+15.4 % Vs 32.8 +30.0 %,P= 0.038) or in OSAC (73.4+15.4 % Vs 1.4+2.2 %, P=0.01). Mean conjunctival epithelial heights were significantly raised in SACS (63.8+9.0 µm) vs controls (44.7+11.2 µm) (P=0.003,unpaired-t-test).
Conclusions: conjunctival epithelial PAR-2 is significantly up-regulated in SAC. This supports the view that disruption of the barrier properties of the conjunctival epithelium is an important event in the pathogenesis of SAC.
1304-1308
Yeoh, S.
db3d0c6c-4b53-4e90-8173-6ae7d5ac8371
Church, M.
dad189d5-866e-4ae1-b005-0d87f74282b8
Lackie, P.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
McGill, J.
31b75610-d63b-42db-9e41-6fde0bb2525b
Mota, M.
420cf738-1abd-409e-b72d-ee284058e679
Hossain, P.
563de5fc-84ad-4539-9228-bde0237eaf51
19 August 2011
Yeoh, S.
db3d0c6c-4b53-4e90-8173-6ae7d5ac8371
Church, M.
dad189d5-866e-4ae1-b005-0d87f74282b8
Lackie, P.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
McGill, J.
31b75610-d63b-42db-9e41-6fde0bb2525b
Mota, M.
420cf738-1abd-409e-b72d-ee284058e679
Hossain, P.
563de5fc-84ad-4539-9228-bde0237eaf51
Yeoh, S., Church, M., Lackie, P., McGill, J., Mota, M. and Hossain, P.
(2011)
Increased conjunctival expression of protease activated receptor 2 (PAR-2) in seasonal allergic conjunctivitis: a role for abnormal conjunctival epithelial permeability in disease pathogenesis?
British Journal of Ophthalmology, 95 (9), .
(doi:10.1136/bjo.2010.191221).
(PMID:21558289)
Abstract
Purpose: aeroallergen exposure to the conjunctival epithelium in seasonal allergic conjunctivitis (SAC) may induce a cellular stress response that disrupts the barrier properties of the conjunctival epithelium resulting in allergic disease. Whether such changes occur in SAC is not known. Epithelial permeability is known to be increased when protease activated receptor 2 (PAR-2) is activated. We evaluated the expression of PAR-2 in patients with SAC in season (SACS) and compared them to control non-atopic subjects or those with out of season allergic conjunctivitis (OSAC).
Methods: 6 SACS, 8 normal and 4 OSAC specimens were examined immunohistochemically for PAR-2 and quantified in a masked fashion for the percentage of epithelia stained for each marker using Image-J software. Conjunctival epithelial heights were measured in all groups to confirm the presence of allergic eye disease.
Results: mean percentage staining of PAR-2 was significantly greater in SACS when compared to normals (73.4+15.4 % Vs 32.8 +30.0 %,P= 0.038) or in OSAC (73.4+15.4 % Vs 1.4+2.2 %, P=0.01). Mean conjunctival epithelial heights were significantly raised in SACS (63.8+9.0 µm) vs controls (44.7+11.2 µm) (P=0.003,unpaired-t-test).
Conclusions: conjunctival epithelial PAR-2 is significantly up-regulated in SAC. This supports the view that disruption of the barrier properties of the conjunctival epithelium is an important event in the pathogenesis of SAC.
This record has no associated files available for download.
More information
e-pub ahead of print date: 10 May 2011
Published date: 19 August 2011
Additional Information:
Originally submitted with the title 'Increased conjunctival expression of PAR-2 in seasonal allergic conjunctivitis: a role for abnormal conjunctival epithelial permeability in disease pathogenesis?'
Identifiers
Local EPrints ID: 181619
URI: http://eprints.soton.ac.uk/id/eprint/181619
ISSN: 0007-1161
PURE UUID: 195cbb61-a536-4ae1-8a87-f625f589557b
Catalogue record
Date deposited: 19 Apr 2011 14:06
Last modified: 15 Mar 2024 03:24
Export record
Altmetrics
Contributors
Author:
S. Yeoh
Author:
M. Church
Author:
J. McGill
Author:
M. Mota
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
