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Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes

Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes
Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes
Background: Characterization of T cell epitopes restricted by common HLA alleles is a powerful tool in the understanding of the immune responses to allergens and for the identification of potential peptides for future peptide immunotherapy (PIT). One important requirement is the identification and use of peptides that will bind to HLA molecules covering a large proportion of the population.

Objective: To identify commonly recognized CD4+ T cell epitopes in Fel d 1, restricted through frequently expressed HLA molecules for potential future use in PIT.

Methods: HLA matched antigen presenting cells, HLA blocking antibodies, and peptide truncations were used in ELISpot assays to establish HLA-restricted T cell epitopes. Cytokine responses were measured by ex vivo and cultured IFN-?, IL-4, and IL-10 ELISpots.

Result: Responses to an immunodominant region of chain 2 were identified in the majority of atopic individuals and epitopes restricted by HLA-DQB1*06 and -DPB1*0401 were characterized in detail. Significantly higher ex vivo IL-4 and lower IFN-? responses were observed to both epitopes in individuals with atopic dermatitis (AD) compared with those without disease. IL-10 responses were significantly lower in those with AD in the individuals with HLA-DPB1*0401.

Conclusions: We have identified an immunodominant region of Fel d 1 which is frequently recognized by CD4+ T cells from atopic individuals and contains epitopes that are restricted by very common HLA alleles.

atopic dermatitis, fel d 1, hla-dpp1*0401, hla-dq b1*06, t cells, t cells epitope
0954-7894
1760-1768
Bateman, E.A.L.
fa517bd7-dad0-4559-b295-32fe4deabd3b
Ardern-Jones, M.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Ogg, G.S.
71330bc0-d3fc-4b8d-a1ea-70c7af7185ae
Bateman, E.A.L.
fa517bd7-dad0-4559-b295-32fe4deabd3b
Ardern-Jones, M.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Ogg, G.S.
71330bc0-d3fc-4b8d-a1ea-70c7af7185ae

Bateman, E.A.L., Ardern-Jones, M. and Ogg, G.S. (2008) Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes. Clinical & Experimental Allergy, 38 (11), 1760-1768. (doi:10.1111/j.1365-2222.2008.03098.x). (PMID:19489915)

Record type: Article

Abstract

Background: Characterization of T cell epitopes restricted by common HLA alleles is a powerful tool in the understanding of the immune responses to allergens and for the identification of potential peptides for future peptide immunotherapy (PIT). One important requirement is the identification and use of peptides that will bind to HLA molecules covering a large proportion of the population.

Objective: To identify commonly recognized CD4+ T cell epitopes in Fel d 1, restricted through frequently expressed HLA molecules for potential future use in PIT.

Methods: HLA matched antigen presenting cells, HLA blocking antibodies, and peptide truncations were used in ELISpot assays to establish HLA-restricted T cell epitopes. Cytokine responses were measured by ex vivo and cultured IFN-?, IL-4, and IL-10 ELISpots.

Result: Responses to an immunodominant region of chain 2 were identified in the majority of atopic individuals and epitopes restricted by HLA-DQB1*06 and -DPB1*0401 were characterized in detail. Significantly higher ex vivo IL-4 and lower IFN-? responses were observed to both epitopes in individuals with atopic dermatitis (AD) compared with those without disease. IL-10 responses were significantly lower in those with AD in the individuals with HLA-DPB1*0401.

Conclusions: We have identified an immunodominant region of Fel d 1 which is frequently recognized by CD4+ T cells from atopic individuals and contains epitopes that are restricted by very common HLA alleles.

Full text not available from this repository.

More information

Published date: November 2008
Keywords: atopic dermatitis, fel d 1, hla-dpp1*0401, hla-dq b1*06, t cells, t cells epitope

Identifiers

Local EPrints ID: 181633
URI: https://eprints.soton.ac.uk/id/eprint/181633
ISSN: 0954-7894
PURE UUID: 4a869e7c-ad27-46b1-9344-ec9b513935d3

Catalogue record

Date deposited: 18 Apr 2011 15:25
Last modified: 16 Jul 2019 23:41

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